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Journal of Virology, March 2004, p. 2642-2647, Vol. 78, No. 5
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.5.2642-2647.2003
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Jaagsiekte Sheep Retrovirus Envelope Efficiently Pseudotypes Human Immunodeficiency Virus Type 1-Based Lentiviral Vectors

Shan-Lu Liu,1,2 Christine L. Halbert,1 and A. Dusty Miller1,2*

Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109,1 Department of Pathology, University of Washington, Seattle, Washington 981952

Received 8 July 2003/ Accepted 13 November 2003

Jaagsiekte sheep retrovirus (JSRV) infects lung epithelial cells in sheep, and oncoretroviral vectors bearing JSRV Env can mediate transduction of human cells, suggesting that such vectors might be useful for lung-directed gene therapy. Here we show that JSRV Env can also efficiently pseudotype a human immunodeficiency virus type 1-based lentiviral vector, a more suitable vector for transduction of slowly dividing lung epithelial cells. We created several chimeric Env proteins that, unlike the parental Env, do not transform rodent fibroblasts but are still capable of pseudotyping lentiviral and oncoretroviral vectors.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Room C2-105, Seattle, WA 98109-1024. Phone: (206) 667-2890. Fax: (206) 667-6523. E-mail: dmiller{at}fhcrc.org.

Journal of Virology, March 2004, p. 2642-2647, Vol. 78, No. 5
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.5.2642-2647.2003
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Link, N., Aubel, C., Kelm, J. M., Marty, R. R., Greber, D., Djonov, V., Bourhis, J., Weber, W., Fussenegger, M. (2006). Therapeutic protein transduction of mammalian cells and mice by nucleic acid-free lentiviral nanoparticles. Nucleic Acids Res 34: e16-e16 [Abstract] [Full Text]  
  • Liu, S.-L., Miller, A. D. (2005). Transformation of Madin-Darby Canine Kidney Epithelial Cells by Sheep Retrovirus Envelope Proteins. J. Virol. 79: 927-933 [Abstract] [Full Text]  


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