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Journal of Virology, February 2004, p. 1954-1961, Vol. 78, No. 4
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.4.1954-1961.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses

Blake Wiedenheft,1,2 Kenneth Stedman,1,3 Francisco Roberto,4 Deborah Willits,1 Anne-Kathrin Gleske,1 Luisa Zoeller,3 Jamie Snyder,1,2 Trevor Douglas,1,5 and Mark Young1,2*

Thermal Biology Institute,1 Department of Microbiology,2 Department of Chemistry, Montana State University, Bozeman, Montana 59717,5 DOE-INEEL, Idaho Falls, Idaho 83415,4 Department of Biology, Portland State University, Portland, Oregon 972013

Received 15 August 2003/ Accepted 20 October 2003

The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined. These nonlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses share the spindle-shaped morphology characteristic of the Fuselloviridae family. These two genomes, in combination with the previously determined SSV1 genome from Japan and the SSV2 genome from Iceland, have allowed us to carry out a phylogenetic comparison of these geographically distributed hyperthermal viruses. Each virus contains a circular double-stranded DNA genome of ~15 kbp with approximately 34 open reading frames (ORFs). These Fusellovirus ORFs show little or no similarity to genes in the public databases. In contrast, 18 ORFs are common to all four isolates and may represent the minimal gene set defining this viral group. In general, ORFs on one half of the genome are colinear and highly conserved, while ORFs on the other half are not. One shared ORF among all four genomes is an integrase of the tyrosine recombinase family. All four viral genomes integrate into their host tRNA genes. The specific tRNA gene used for integration varies, and one genome integrates into multiple loci. Several unique ORFs are found in the genome of each isolate.

* Corresponding author. Mailing address: Montana State University, Dept. of Plant Sciences and Plant Pathology, P.O. Box 173150, Bozeman, MT 59717. Phone: (406) 994-5158. Fax: (406) 994-7600. E-mail: myoung{at}montana.edu.

Journal of Virology, February 2004, p. 1954-1961, Vol. 78, No. 4
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.4.1954-1961.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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