This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maurer, K.
Right arrow Articles by Rümenapf, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maurer, K.
Right arrow Articles by Rümenapf, T.

 Previous Article  |  Next Article 

Journal of Virology, February 2004, p. 1792-1799, Vol. 78, No. 4
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.4.1792-1799.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

CD46 Is a Cellular Receptor for Bovine Viral Diarrhea Virus

Karin Maurer,1 Thomas Krey,1 Volker Moennig,2 Heinz-Jürgen Thiel,1 and Till Rümenapf1*

Institut für Virologie, Justus-Liebig-Universität, 35392 Giessen,1 Institut für Virologie, Tierärztliche Hochschule Hannover, 30559 Hannover, Germany2

Received 21 July 2003/ Accepted 30 October 2003

Various monoclonal antibodies (MAbs) that recognize cell surface proteins on bovine cells were previously shown to efficiently block infection with bovine viral diarrhea virus (BVDV) (C. Schelp, I. Greiser-Wilke, G. Wolf, M. Beer, V. Moennig, and B. Liess, Arch. Virol. 140:1997-2009, 1995). With one of these MAbs, a 50- to 58-kDa protein was purified from calf thymus by immunoaffinity chromatography. Microchemical analysis of two internal peptides revealed significant sequence homology to porcine and human CD46. The cDNA of bovine CD46 (CD46bov) was cloned and further characterized. Heterologously expressed CD46bov was detected by the MAb used for purification. A putative function of CD46bov as a BVDV receptor was studied with respect to virus binding and susceptibility of nonpermissive cells. While the expression of CD46bov correlated well with the binding of [3H]uridine-labeled BVDV, the susceptibility of cells nonpermissive for BVDV was not observed. However, the expression of CD46bov resulted in a significant increase in the susceptibility of porcine cells to BVDV. These results provide strong evidence that CD46bov serves as a cellular receptor for BVDV.

* Corresponding author. Mailing address: Institut für Virologie, Frankfurter Str. 107, 35392 Giessen, Germany. Phone: 49-641-99-38356. Fax: 49-641-99-38359. E-mail: Till.H.Ruemenapf{at}vetmed.uni-giessen.de.

Journal of Virology, February 2004, p. 1792-1799, Vol. 78, No. 4
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.4.1792-1799.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tscherne, D. M., Evans, M. J., MacDonald, M. R., Rice, C. M. (2008). Transdominant Inhibition of Bovine Viral Diarrhea Virus Entry. J. Virol. 82: 2427-2436 [Abstract] [Full Text]  
  • Krey, T., Moussay, E., Thiel, H.-J., Rumenapf, T. (2006). Role of the Low-Density Lipoprotein Receptor in Entry of Bovine Viral Diarrhea Virus. J. Virol. 80: 10862-10867 [Abstract] [Full Text]  
  • Krey, T., Himmelreich, A., Heimann, M., Menge, C., Thiel, H.-J., Maurer, K., Rumenapf, T. (2006). Function of Bovine CD46 as a Cellular Receptor for Bovine Viral Diarrhea Virus Is Determined by Complement Control Protein 1.. J. Virol. 80: 3912-3922 [Abstract] [Full Text]  
  • Heimann, M., Sosa, G. R., Martoglio, B., Thiel, H.-J., Rumenapf, T. (2006). Core Protein of Pestiviruses Is Processed at the C Terminus by Signal Peptide Peptidase. J. Virol. 80: 1915-1921 [Abstract] [Full Text]  
  • Fetzer, C., Tews, B. A., Meyers, G. (2005). The Carboxy-Terminal Sequence of the Pestivirus Glycoprotein Erns Represents an Unusual Type of Membrane Anchor. J. Virol. 79: 11901-11913 [Abstract] [Full Text]  
  • Lecot, S., Belouzard, S., Dubuisson, J., Rouille, Y. (2005). Bovine Viral Diarrhea Virus Entry Is Dependent on Clathrin-Mediated Endocytosis. J. Virol. 79: 10826-10829 [Abstract] [Full Text]  
  • Fleischli, C., Verhaagh, S., Havenga, M., Sirena, D., Schaffner, W., Cattaneo, R., Greber, U. F., Hemmi, S. (2005). The Distal Short Consensus Repeats 1 and 2 of the Membrane Cofactor Protein CD46 and Their Distance from the Cell Membrane Determine Productive Entry of Species B Adenovirus Serotype 35. J. Virol. 79: 10013-10022 [Abstract] [Full Text]  
  • Gaggar, A., Shayakhmetov, D. M., Liszewski, M. K., Atkinson, J. P., Lieber, A. (2005). Localization of Regions in CD46 That Interact with Adenovirus. J. Virol. 79: 7503-7513 [Abstract] [Full Text]  
  • Krey, T., Thiel, H.-J., Rumenapf, T. (2005). Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry. J. Virol. 79: 4191-4200 [Abstract] [Full Text]  
  • Lee, Y.-M., Tscherne, D. M., Yun, S.-I., Frolov, I., Rice, C. M. (2005). Dual Mechanisms of Pestiviral Superinfection Exclusion at Entry and RNA Replication. J. Virol. 79: 3231-3242 [Abstract] [Full Text]  
  • Kitagawa, Y., Tani, H., Limn, C. K., Matsunaga, T. M., Moriishi, K., Matsuura, Y. (2005). Ligand-Directed Gene Targeting to Mammalian Cells by Pseudotype Baculoviruses. J. Virol. 79: 3639-3652 [Abstract] [Full Text]  
  • Cattaneo, R. (2004). Four Viruses, Two Bacteria, and One Receptor: Membrane Cofactor Protein (CD46) as Pathogens' Magnet. J. Virol. 78: 4385-4388 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»