This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakayama, T.
Right arrow Articles by Yoshie, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakayama, T.
Right arrow Articles by Yoshie, O.

 Previous Article  |  Next Article 

Journal of Virology, February 2004, p. 1665-1674, Vol. 78, No. 4
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.4.1665-1674.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Selective Induction of Th2-Attracting Chemokines CCL17 and CCL22 in Human B Cells by Latent Membrane Protein 1 of Epstein-Barr Virus

Takashi Nakayama,1 Kunio Hieshima,1 Daisuke Nagakubo,1 Emiko Sato,2 Masahiro Nakayama,2 Keisei Kawa,2 and Osamu Yoshie1*

Department of Microbiology, Kinki University School of Medicine, Osaka-Sayama, Osaka 589-8511,1 Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi City, Osaka 594-1101, Japan2

Received 11 August 2003/ Accepted 27 October 2003

Chemokines are likely to play important roles in the pathophysiology of diseases associated with Epstein-Barr virus (EBV). Here, we have analyzed the repertoire of chemokines expressed by EBV-infected B cells. EBV infection of B cells induced expression of TARC/CCL17 and MDC/CCL22, which are known to attract Th2 cells and regulatory T cells via CCR4, and also upregulated constitutive expression of MIP-1{alpha}/CCL3, MIP-1ß/CCL4, and RANTES/CCL5, which are known to attract Th1 cells and cytotoxic T cells via CCR5. Accordingly, EBV-immortalized B cells secreted these chemokines, especially CCL3, CCL4, and CCL22, in large quantities. EBV infection or stable expression of LMP1 also induced CCL17 and CCL22 in a B-cell line, BJAB. The inhibitors of the TRAF/NF-{kappa}B pathway (BAY11-7082) and the p38/ATF2 pathway (SB202190) selectively suppressed the expression of CCL17 and CCL22 in EBV-immortalized B cells and BJAB-LMP1. Consistently, transient-transfection assays using CCL22 promoter-reporter constructs demonstrated that two NF-{kappa}B sites and a single AP-1 site were involved in the activation of the CCL22 promoter by LMP1. Finally, serum CCL22 levels were significantly elevated in infectious mononucleosis. Collectively, LMP1 induces CCL17 and CCL22 in EBV-infected B cells via activation of NF-{kappa}B and probably ATF2. Production of CCL17 and CCL22, which attract Th2 and regulatory T cells, may help EBV-infected B cells evade immune surveillance by Th1 cells. However, the concomitant production of CCL3, CCL4, and CCL5 by EBV-infected B cells may eventually attract Th1 cells and cytotoxic T cells, leading to elimination of EBV-infected B cells at latency III and to selection of those with limited expression of latent genes.

* Corresponding author. Mailing address: Department of Microbiology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan. Phone and fax: 81-(0)723-67-3606. E-mail: o.yoshie{at}med.kindai.ac.jp.

Journal of Virology, February 2004, p. 1665-1674, Vol. 78, No. 4
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.4.1665-1674.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Poole, E., Groves, I., Macdonald, A., Pang, Y., Alcami, A., Sinclair, J. (2009). Identification of TRIM23 as a Cofactor Involved in the Regulation of NF-{kappa}B by Human Cytomegalovirus. J. Virol. 83: 3581-3590 [Abstract] [Full Text]  
  • Pokharel, Y. R., Lim, S. C., Kim, S. C., Heo, T.-H., Choi, H. K., Kang, K. W. (2008). Sopungyangjae-Tang Inhibits Development of Dermatitis in Nc/Nga Mice. Evid Based Complement Alternat Med 5: 173-180 [Abstract] [Full Text]  
  • Poole, E., Atkins, E., Nakayama, T., Yoshie, O., Groves, I., Alcami, A., Sinclair, J. (2008). NF-{kappa}B-Mediated Activation of the Chemokine CCL22 by the Product of the Human Cytomegalovirus Gene UL144 Escapes Regulation by Viral IE86. J. Virol. 82: 4250-4256 [Abstract] [Full Text]  
  • Hieshima, K., Nagakubo, D., Nakayama, T., Shirakawa, A.-K., Jin, Z., Yoshie, O. (2008). Tax-Inducible Production of CC Chemokine Ligand 22 by Human T Cell Leukemia Virus Type 1 (HTLV-1)-Infected T Cells Promotes Preferential Transmission of HTLV-1 to CCR4-Expressing CD4+ T Cells. J. Immunol. 180: 931-939 [Abstract] [Full Text]  
  • Haas, J., Schopp, L., Storch-Hagenlocher, B., Fritzsching, B., Jacobi, C., Milkova, L., Fritz, B., Schwarz, A., Suri-Payer, E., Hensel, M., Wildemann, B. (2008). Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis. Blood 111: 761-766 [Abstract] [Full Text]  
  • Heijink, I. H., Kies, P. M., Kauffman, H. F., Postma, D. S., van Oosterhout, A. J. M., Vellenga, E. (2007). Down-Regulation of E-Cadherin in Human Bronchial Epithelial Cells Leads to Epidermal Growth Factor Receptor-Dependent Th2 Cell-Promoting Activity. J. Immunol. 178: 7678-7685 [Abstract] [Full Text]  
  • Tadagaki, K., Yamanishi, K., Mori, Y. (2007). Reciprocal roles of cellular chemokine receptors and human herpesvirus 7-encoded chemokine receptors, U12 and U51. J. Gen. Virol. 88: 1423-1428 [Abstract] [Full Text]  
  • Penna, G., Amuchastegui, S., Giarratana, N., Daniel, K. C., Vulcano, M., Sozzani, S., Adorini, L. (2007). 1,25-Dihydroxyvitamin D3 Selectively Modulates Tolerogenic Properties in Myeloid but Not Plasmacytoid Dendritic Cells. J. Immunol. 178: 145-153 [Abstract] [Full Text]  
  • Ishida, T., Ishii, T., Inagaki, A., Yano, H., Komatsu, H., Iida, S., Inagaki, H., Ueda, R. (2006). Specific Recruitment of CC Chemokine Receptor 4-Positive Regulatory T Cells in Hodgkin Lymphoma Fosters Immune Privilege. Cancer Res. 66: 5716-5722 [Abstract] [Full Text]  
  • Ettmayer, P., Mayer, P., Kalthoff, F., Neruda, W., Harrer, N., Hartmann, G., Epstein, M. M., Brinkmann, V., Heusser, C., Woisetschlager, M. (2006). A Novel Low Molecular Weight Inhibitor of Dendritic Cells and B Cells Blocks Allergic Inflammation. Am. J. Respir. Crit. Care Med. 173: 599-606 [Abstract] [Full Text]  
  • Lee, B.-S., Lee, S.-H., Feng, P., Chang, H., Cho, N.-H., Jung, J. U. (2005). Characterization of the Kaposi's Sarcoma-Associated Herpesvirus K1 Signalosome. J. Virol. 79: 12173-12184 [Abstract] [Full Text]  
  • Ghadially, H., Ross, X.-L., Kerst, C., Dong, J., Reske-Kunz, A. B., Ross, R. (2005). Differential Regulation of CCL22 Gene Expression in Murine Dendritic Cells and B Cells. J. Immunol. 174: 5620-5629 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»