This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, J.
Right arrow Articles by McKeating, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, J.
Right arrow Articles by McKeating, J. A.

 Previous Article  |  Next Article 

Journal of Virology, February 2004, p. 1448-1455, Vol. 78, No. 3
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.3.1448-1455.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

CD81 Is Required for Hepatitis C Virus Glycoprotein-Mediated Viral Infection

Jie Zhang,1 Glenn Randall,1 Adrian Higginbottom,2 Peter Monk,2 Charles M. Rice,1 and Jane A. McKeating1*

Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, New York 10021,1 School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield S10 2RX, United Kingdom2

Received 31 July 2003/ Accepted 4 October 2003

CD81 has been described as a putative receptor for hepatitis C virus (HCV); however, its role in HCV cell entry has not been characterized due to the lack of an efficient cell culture system. We have examined the role of CD81 in HCV glycoprotein-dependent entry by using a recently developed retroviral pseudotyping system. Human immunodeficiency virus (HIV) pseudotypes bearing HCV E1E2 glycoproteins show a restricted tropism for human liver cell lines. Although all of the permissive cell lines express CD81, CD81 expression alone is not sufficient to allow viral entry. CD81 is required for HIV-HCV pseudotype infection since (i) a monoclonal antibody specific for CD81 inhibited infection of susceptible target cells and (ii) silencing of CD81 expression in Huh-7.5 hepatoma cells by small interfering RNAs inhibited HIV-HCV pseudotype infection. Furthermore, expression of CD81 in human liver cells that were previously resistant to infection, HepG2 and HH29, conferred permissivity of HCV pseudotype infection. The characterization of chimeric CD9/CD81 molecules confirmed that the large extracellular loop of CD81 is a determinant for viral entry. These data suggest a functional role for CD81 as a coreceptor for HCV glycoprotein-dependent viral cell entry.

* Corresponding author. Mailing address: Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-7066. Fax: (212) 327-7048. E-mail: mckeatj{at}rockefeller.edu.

Journal of Virology, February 2004, p. 1448-1455, Vol. 78, No. 3
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.3.1448-1455.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schwarz, A. K., Grove, J., Hu, K., Mee, C. J., Balfe, P., McKeating, J. A. (2009). Hepatoma Cell Density Promotes Claudin-1 and Scavenger Receptor BI Expression and Hepatitis C Virus Internalization. J. Virol. 83: 12407-12414 [Abstract] [Full Text]  
  • Weng, J., Krementsov, D. N., Khurana, S., Roy, N. H., Thali, M. (2009). Formation of Syncytia Is Repressed by Tetraspanins in Human Immunodeficiency Virus Type 1-Producing Cells. J. Virol. 83: 7467-7474 [Abstract] [Full Text]  
  • Berger, K. L., Cooper, J. D., Heaton, N. S., Yoon, R., Oakland, T. E., Jordan, T. X., Mateu, G., Grakoui, A., Randall, G. (2009). Roles for endocytic trafficking and phosphatidylinositol 4-kinase III alpha in hepatitis C virus replication. Proc. Natl. Acad. Sci. USA 106: 7577-7582 [Abstract] [Full Text]  
  • Burlone, M. E., Budkowska, A. (2009). Hepatitis C virus cell entry: role of lipoproteins and cellular receptors. J. Gen. Virol. 90: 1055-1070 [Abstract] [Full Text]  
  • Timpe, J M, McKeating, J A (2008). Hepatitis C virus entry: possible targets for therapy. Gut 57: 1728-1737 [Full Text]  
  • Farquhar, M. J., Harris, H. J., Diskar, M., Jones, S., Mee, C. J., Nielsen, S. U., Brimacombe, C. L., Molina, S., Toms, G. L., Maurel, P., Howl, J., Herberg, F. W., van IJzendoorn, S. C. D., Balfe, P., McKeating, J. A. (2008). Protein Kinase A-Dependent Step(s) in Hepatitis C Virus Entry and Infectivity. J. Virol. 82: 8797-8811 [Abstract] [Full Text]  
  • Harris, H. J., Farquhar, M. J., Mee, C. J., Davis, C., Reynolds, G. M., Jennings, A., Hu, K., Yuan, F., Deng, H., Hubscher, S. G., Han, J. H., Balfe, P., McKeating, J. A. (2008). CD81 and Claudin 1 Coreceptor Association: Role in Hepatitis C Virus Entry. J. Virol. 82: 5007-5020 [Abstract] [Full Text]  
  • Jones, M., Owen, J. S (2008). How does interferon inhibit HCV cell entry?. Gut 57: 573-574 [Full Text]  
  • Yang, W., Qiu, C., Biswas, N., Jin, J., Watkins, S. C., Montelaro, R. C., Coyne, C. B., Wang, T. (2008). Correlation of the Tight Junction-like Distribution of Claudin-1 to the Cellular Tropism of Hepatitis C Virus. J. Biol. Chem. 283: 8643-8653 [Abstract] [Full Text]  
  • von Hahn, T., Rice, C. M. (2008). Hepatitis C Virus Entry. J. Biol. Chem. 283: 3689-3693 [Full Text]  
  • Zheng, A., Yuan, F., Li, Y., Zhu, F., Hou, P., Li, J., Song, X., Ding, M., Deng, H. (2007). Claudin-6 and Claudin-9 Function as Additional Coreceptors for Hepatitis C Virus. J. Virol. 81: 12465-12471 [Abstract] [Full Text]  
  • Dreux, M., Boson, B., Ricard-Blum, S., Molle, J., Lavillette, D., Bartosch, B., Pecheur, E.-I., Cosset, F.-L. (2007). The Exchangeable Apolipoprotein ApoC-I Promotes Membrane Fusion of Hepatitis C Virus. J. Biol. Chem. 282: 32357-32369 [Abstract] [Full Text]  
  • Tellinghuisen, T. L., Evans, M. J., von Hahn, T., You, S., Rice, C. M. (2007). Studying Hepatitis C Virus: Making the Best of a Bad Virus. J. Virol. 81: 8853-8867 [Full Text]  
  • Tani, H., Komoda, Y., Matsuo, E., Suzuki, K., Hamamoto, I., Yamashita, T., Moriishi, K., Fujiyama, K., Kanto, T., Hayashi, N., Owsianka, A., Patel, A. H., Whitt, M. A., Matsuura, Y. (2007). Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins. J. Virol. 81: 8601-8612 [Abstract] [Full Text]  
  • Catanese, M. T., Graziani, R., von Hahn, T., Moreau, M., Huby, T., Paonessa, G., Santini, C., Luzzago, A., Rice, C. M., Cortese, R., Vitelli, A., Nicosia, A. (2007). High-Avidity Monoclonal Antibodies against the Human Scavenger Class B Type I Receptor Efficiently Block Hepatitis C Virus Infection in the Presence of High-Density Lipoprotein. J. Virol. 81: 8063-8071 [Abstract] [Full Text]  
  • Falkowska, E., Kajumo, F., Garcia, E., Reinus, J., Dragic, T. (2007). Hepatitis C Virus Envelope Glycoprotein E2 Glycans Modulate Entry, CD81 Binding, and Neutralization. J. Virol. 81: 8072-8079 [Abstract] [Full Text]  
  • Randall, G., Panis, M., Cooper, J. D., Tellinghuisen, T. L., Sukhodolets, K. E., Pfeffer, S., Landthaler, M., Landgraf, P., Kan, S., Lindenbach, B. D., Chien, M., Weir, D. B., Russo, J. J., Ju, J., Brownstein, M. J., Sheridan, R., Sander, C., Zavolan, M., Tuschl, T., Rice, C. M. (2007). Cellular cofactors affecting hepatitis C virus infection and replication. Proc. Natl. Acad. Sci. USA 104: 12884-12889 [Abstract] [Full Text]  
  • Cai, Z., Cai, L., Jiang, J., Chang, K.-S., van der Westhuyzen, D. R., Luo, G. (2007). Human Serum Amyloid A Protein Inhibits Hepatitis C Virus Entry into Cells. J. Virol. 81: 6128-6133 [Abstract] [Full Text]  
  • Akazawa, D., Date, T., Morikawa, K., Murayama, A., Miyamoto, M., Kaga, M., Barth, H., Baumert, T. F., Dubuisson, J., Wakita, T. (2007). CD81 Expression Is Important for the Permissiveness of Huh7 Cell Clones for Heterogeneous Hepatitis C Virus Infection. J. Virol. 81: 5036-5045 [Abstract] [Full Text]  
  • Tscherne, D. M., Evans, M. J., von Hahn, T., Jones, C. T., Stamataki, Z., McKeating, J. A., Lindenbach, B. D., Rice, C. M. (2007). Superinfection Exclusion in Cells Infected with Hepatitis C Virus. J. Virol. 81: 3693-3703 [Abstract] [Full Text]  
  • Grove, J., Huby, T., Stamataki, Z., Vanwolleghem, T., Meuleman, P., Farquhar, M., Schwarz, A., Moreau, M., Owen, J. S., Leroux-Roels, G., Balfe, P., McKeating, J. A. (2007). Scavenger Receptor BI and BII Expression Levels Modulate Hepatitis C Virus Infectivity. J. Virol. 81: 3162-3169 [Abstract] [Full Text]  
  • Brown, R. J. P., Tarr, A. W., McClure, C. P., Juttla, V. S., Tagiuri, N., Irving, W. L., Ball, J. K. (2007). Cross-genotype characterization of genetic diversity and molecular adaptation in hepatitis C virus envelope glycoprotein genes. J. Gen. Virol. 88: 458-469 [Abstract] [Full Text]  
  • Koutsoudakis, G., Herrmann, E., Kallis, S., Bartenschlager, R., Pietschmann, T. (2007). The Level of CD81 Cell Surface Expression Is a Key Determinant for Productive Entry of Hepatitis C Virus into Host Cells. J. Virol. 81: 588-598 [Abstract] [Full Text]  
  • Kapadia, S. B., Barth, H., Baumert, T., McKeating, J. A., Chisari, F. V. (2007). Initiation of Hepatitis C Virus Infection Is Dependent on Cholesterol and Cooperativity between CD81 and Scavenger Receptor B Type I. J. Virol. 81: 374-383 [Abstract] [Full Text]  
  • Flint, M., von Hahn, T., Zhang, J., Farquhar, M., Jones, C. T., Balfe, P., Rice, C. M., McKeating, J. A. (2006). Diverse CD81 Proteins Support Hepatitis C Virus Infection. J. Virol. 80: 11331-11342 [Abstract] [Full Text]  
  • Zhong, J., Gastaminza, P., Chung, J., Stamataki, Z., Isogawa, M., Cheng, G., McKeating, J. A., Chisari, F. V. (2006). Persistent Hepatitis C Virus Infection In Vitro: Coevolution of Virus and Host. J. Virol. 80: 11082-11093 [Abstract] [Full Text]  
  • Codran, A., Royer, C., Jaeck, D., Bastien-Valle, M., Baumert, T. F., Kieny, M. P., Pereira, C. A., Martin, J.-P. (2006). Entry of hepatitis C virus pseudotypes into primary human hepatocytes by clathrin-dependent endocytosis. J. Gen. Virol. 87: 2583-2593 [Abstract] [Full Text]  
  • Owsianka, A. M., Timms, J. M., Tarr, A. W., Brown, R. J. P., Hickling, T. P., Szwejk, A., Bienkowska-Szewczyk, K., Thomson, B. J., Patel, A. H., Ball, J. K. (2006). Identification of conserved residues in the e2 envelope glycoprotein of the hepatitis C virus that are critical for CD81 binding.. J. Virol. 80: 8695-8704 [Abstract] [Full Text]  
  • Drummer, H. E., Boo, I., Maerz, A. L., Poumbourios, P. (2006). A conserved gly436-trp-leu-ala-gly-leu-phe-tyr motif in hepatitis C virus glycoprotein e2 is a determinant of CD81 binding and viral entry.. J. Virol. 80: 7844-7853 [Abstract] [Full Text]  
  • Kronenberger, B., Herrmann, E., Hofmann, W. P., Wedemeyer, H., Sester, M., Mihm, U., Ghaliai, T., Zeuzem, S., Sarrazin, C. (2006). Dynamics of CD81 expression on lymphocyte subsets during interferon-{alpha}-based antiviral treatment of patients with chronic hepatitis C. J. Leukoc. Biol. 80: 298-308 [Abstract] [Full Text]  
  • Zhao, L.-J., Zhang, X.-L., Zhao, P., Cao, J., Cao, M.-M., Zhu, S.-Y., Liu, H.-Q., Qi, Z.-T. (2006). Up-regulation of ERK and p38 MAPK signaling pathways by hepatitis C virus E2 envelope protein in human T lymphoma cell line. J. Leukoc. Biol. 80: 424-432 [Abstract] [Full Text]  
  • Dreux, M., Pietschmann, T., Granier, C., Voisset, C., Ricard-Blum, S., Mangeot, P.-E., Keck, Z., Foung, S., Vu-Dac, N., Dubuisson, J., Bartenschlager, R., Lavillette, D., Cosset, F.-L. (2006). High Density Lipoprotein Inhibits Hepatitis C Virus-neutralizing Antibodies by Stimulating Cell Entry via Activation of the Scavenger Receptor BI. J. Biol. Chem. 281: 18285-18295 [Abstract] [Full Text]  
  • Lai, W. K., Sun, P. J., Zhang, J., Jennings, A., Lalor, P. F., Hubscher, S., McKeating, J. A., Adams, D. H. (2006). Expression of DC-SIGN and DC-SIGNR on Human Sinusoidal Endothelium: A Role for Capturing Hepatitis C Virus Particles. Am. J. Pathol. 169: 200-208 [Abstract] [Full Text]  
  • Koutsoudakis, G., Kaul, A., Steinmann, E., Kallis, S., Lohmann, V., Pietschmann, T., Bartenschlager, R. (2006). Characterization of the early steps of hepatitis C virus infection by using luciferase reporter viruses.. J. Virol. 80: 5308-5320 [Abstract] [Full Text]  
  • Silvie, O., Charrin, S., Billard, M., Franetich, J.-F., Clark, K. L., van Gemert, G.-J., Sauerwein, R. W., Dautry, F., Boucheix, C., Mazier, D., Rubinstein, E. (2006). Cholesterol contributes to the organization of tetraspanin-enriched microdomains and to CD81-dependent infection by malaria sporozoites. J. Cell Sci. 119: 1992-2002 [Abstract] [Full Text]  
  • Cocquerel, L., Voisset, C., Dubuisson, J. (2006). Hepatitis C virus entry: potential receptors and their biological functions.. J. Gen. Virol. 87: 1075-1084 [Abstract] [Full Text]  
  • Bertaux, C., Dragic, T. (2006). Different domains of CD81 mediate distinct stages of hepatitis C virus pseudoparticle entry.. J. Virol. 80: 4940-4948 [Abstract] [Full Text]  
  • Lavillette, D., Bartosch, B., Nourrisson, D., Verney, G., Cosset, F.-L., Penin, F., Pecheur, E.-I. (2006). Hepatitis C Virus Glycoproteins Mediate Low pH-dependent Membrane Fusion with Liposomes. J. Biol. Chem. 281: 3909-3917 [Abstract] [Full Text]  
  • Tscherne, D. M., Jones, C. T., Evans, M. J., Lindenbach, B. D., McKeating, J. A., Rice, C. M. (2006). Time- and Temperature-Dependent Activation of Hepatitis C Virus for Low-pH-Triggered Entry. J. Virol. 80: 1734-1741 [Abstract] [Full Text]  
  • Kobayashi, M., Bennett, M. C., Bercot, T., Singh, I. R. (2006). Functional Analysis of Hepatitis C Virus Envelope Proteins, Using a Cell-Cell Fusion Assay. J. Virol. 80: 1817-1825 [Abstract] [Full Text]  
  • Callens, N., Ciczora, Y., Bartosch, B., Vu-Dac, N., Cosset, F.-L., Pawlotsky, J.-M., Penin, F., Dubuisson, J. (2005). Basic Residues in Hypervariable Region 1 of Hepatitis C Virus Envelope Glycoprotein E2 Contribute to Virus Entry. J. Virol. 79: 15331-15341 [Abstract] [Full Text]  
  • Cai, Z., Zhang, C., Chang, K.-S., Jiang, J., Ahn, B.-C., Wakita, T., Liang, T. J., Luo, G. (2005). Robust Production of Infectious Hepatitis C Virus (HCV) from Stably HCV cDNA-Transfected Human Hepatoma Cells. J. Virol. 79: 13963-13973 [Abstract] [Full Text]  
  • Yamada, E., Montoya, M., Schuettler, C. G., Hickling, T. P., Tarr, A. W., Vitelli, A., Dubuisson, J., Patel, A. H., Ball, J. K., Borrow, P. (2005). Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets. J. Gen. Virol. 86: 2507-2512 [Abstract] [Full Text]  
  • Martin, F., Roth, D. M., Jans, D. A., Pouton, C. W., Partridge, L. J., Monk, P. N., Moseley, G. W. (2005). Tetraspanins in Viral Infections: a Fundamental Role in Viral Biology?. J. Virol. 79: 10839-10851 [Full Text]  
  • Owsianka, A., Tarr, A. W., Juttla, V. S., Lavillette, D., Bartosch, B., Cosset, F.-L., Ball, J. K., Patel, A. H. (2005). Monoclonal Antibody AP33 Defines a Broadly Neutralizing Epitope on the Hepatitis C Virus E2 Envelope Glycoprotein. J. Virol. 79: 11095-11104 [Abstract] [Full Text]  
  • Lindenbach, B. D., Evans, M. J., Syder, A. J., Wolk, B., Tellinghuisen, T. L., Liu, C. C., Maruyama, T., Hynes, R. O., Burton, D. R., McKeating, J. A., Rice, C. M. (2005). Complete Replication of Hepatitis C Virus in Cell Culture. Science 309: 623-626 [Abstract] [Full Text]  
  • Machida, K., Cheng, K. T.-H., Pavio, N., Sung, V. M.-H., Lai, M. M. C. (2005). Hepatitis C Virus E2-CD81 Interaction Induces Hypermutation of the Immunoglobulin Gene in B Cells. J. Virol. 79: 8079-8089 [Abstract] [Full Text]  
  • Bartosch, B., Verney, G., Dreux, M., Donot, P., Morice, Y., Penin, F., Pawlotsky, J.-M., Lavillette, D., Cosset, F.-L. (2005). An Interplay between Hypervariable Region 1 of the Hepatitis C Virus E2 Glycoprotein, the Scavenger Receptor BI, and High-Density Lipoprotein Promotes both Enhancement of Infection and Protection against Neutralizing Antibodies. J. Virol. 79: 8217-8229 [Abstract] [Full Text]  
  • Goffard, A., Callens, N., Bartosch, B., Wychowski, C., Cosset, F.-L., Montpellier, C., Dubuisson, J. (2005). Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Glycoproteins. J. Virol. 79: 8400-8409 [Abstract] [Full Text]  
  • Zhong, J., Gastaminza, P., Cheng, G., Kapadia, S., Kato, T., Burton, D. R., Wieland, S. F., Uprichard, S. L., Wakita, T., Chisari, F. V. (2005). Robust hepatitis C virus infection in vitro. Proc. Natl. Acad. Sci. USA 102: 9294-9299 [Abstract] [Full Text]  
  • Barth, H., Cerino, R., Arcuri, M., Hoffmann, M., Schurmann, P., Adah, M. I., Gissler, B., Zhao, X., Ghisetti, V., Lavezzo, B., Blum, H. E., von Weizsacker, F., Vitelli, A., Scarselli, E., Baumert, T. F. (2005). Scavenger Receptor Class B Type I and Hepatitis C Virus Infection of Primary Tupaia Hepatocytes. J. Virol. 79: 5774-5785 [Abstract] [Full Text]  
  • Toledo, M. S., Suzuki, E., Handa, K., Hakomori, S. (2005). Effect of Ganglioside and Tetraspanins in Microdomains on Interaction of Integrins with Fibroblast Growth Factor Receptor. J. Biol. Chem. 280: 16227-16234 [Abstract] [Full Text]  
  • Voisset, C., Callens, N., Blanchard, E., Op De Beeck, A., Dubuisson, J., Vu-Dac, N. (2005). High Density Lipoproteins Facilitate Hepatitis C Virus Entry through the Scavenger Receptor Class B Type I. J. Biol. Chem. 280: 7793-7799 [Abstract] [Full Text]  
  • Meyer, K., Beyene, A., Bowlin, T. L., Basu, A., Ray, R. (2004). Coexpression of Hepatitis C Virus E1 and E2 Chimeric Envelope Glycoproteins Displays Separable Ligand Sensitivity and Increases Pseudotype Infectious Titer. J. Virol. 78: 12838-12847 [Abstract] [Full Text]  
  • Cormier, E. G., Durso, R. J., Tsamis, F., Boussemart, L., Manix, C., Olson, W. C., Gardner, J. P., Dragic, T. (2004). L-SIGN (CD209L) and DC-SIGN (CD209) mediate transinfection of liver cells by hepatitis C virus. Proc. Natl. Acad. Sci. USA 101: 14067-14072 [Abstract] [Full Text]  
  • Keck, Z.-Y., Op De Beeck, A., Hadlock, K. G., Xia, J., Li, T.-K., Dubuisson, J., Foung, S. K. H. (2004). Hepatitis C Virus E2 Has Three Immunogenic Domains Containing Conformational Epitopes with Distinct Properties and Biological Functions. J. Virol. 78: 9224-9232 [Abstract] [Full Text]  
  • McKeating, J. A., Zhang, L. Q., Logvinoff, C., Flint, M., Zhang, J., Yu, J., Butera, D., Ho, D. D., Dustin, L. B., Rice, C. M., Balfe, P. (2004). Diverse Hepatitis C Virus Glycoproteins Mediate Viral Infection in a CD81-Dependent Manner. J. Virol. 78: 8496-8505 [Abstract] [Full Text]  
  • Drummer, H. E., Poumbourios, P. (2004). Hepatitis C Virus Glycoprotein E2 Contains a Membrane-proximal Heptad Repeat Sequence That Is Essential for E1E2 Glycoprotein Heterodimerization and Viral Entry. J. Biol. Chem. 279: 30066-30072 [Abstract] [Full Text]  
  • Flint, M., Logvinoff, C., Rice, C. M., McKeating, J. A. (2004). Characterization of Infectious Retroviral Pseudotype Particles Bearing Hepatitis C Virus Glycoproteins. J. Virol. 78: 6875-6882 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»