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Journal of Virology, December 2004, p. 14066-14069, Vol. 78, No. 24
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.24.14066-14069.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Infectious Molecular Clone of a Recently Transmitted Pediatric Human Immunodeficiency Virus Clade C Isolate from Africa: Evidence of Intraclade Recombination

Ricky D. Grisson,1,2 Agnès-Laurence Chenine,1,2 Lan-Yu Yeh,1 Jun He,3 Charles Wood,3 Ganapati J. Bhat,4 Weidong Xu,1,2 Chipepo Kankasa,4 and Ruth M. Ruprecht1,2*

Department of Cancer, Immunology and AIDS, Dana-Farber Cancer Institute,1 Department of Medicine, Harvard Medical School, Boston, Massachusetts,2 Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln, Nebraska,3 Department of Pediatrics, University Teaching Hospital, Lusaka, Zambia4

Received 18 September 2003/ Accepted 2 June 2004

Although human immunodeficiency virus type 1 (HIV-1) clade C continues to dominate the pandemic, only two infectious clade C proviral DNA clones have been described (N. Mochizuki, N. Otsuka, K. Matsuo, T. Shiino, A. Kojima, T. Kurata, K. Sakai, N. Yamamoto, S. Isomura, T. N. Dhole, Y. Takebe, M. Matsuda, and M. Tatsumi, AIDS Res. Hum. Retrovir. 15:1321-1324, 1999; T. Ndung'u, B. Renjifo, and M. Essex, J. Virol. 75:4964-4972, 2001). We have generated an infectious molecular clone of a pediatric clade C strain, HIV1084i, which was isolated from a Zambian infant infected either intrapartum or through breastfeeding. HIV1084i is an R5, non-syncytium-inducing isolate that bears all known clade C signatures; gag, pol, and env consistently mapped within clade C. Interestingly, gag resembled Asian isolates, whereas pol and env resembled African isolates, indicating that HIV1084i probably arose from an intraclade recombination. As a recently transmitted clade C strain, HIV1084i will be a useful vaccine development tool.

* Corresponding author. Mailing address: Dana-Farber Cancer Institute, 44 Binney St., Boston, MA 02115-6084. Phone: (617) 632-3719. Fax: (617) 632-3112. E-mail: ruth_ruprecht{at}dfci.harvard.edu.

Journal of Virology, December 2004, p. 14066-14069, Vol. 78, No. 24
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.24.14066-14069.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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