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Journal of Virology, December 2004, p. 14039-14042, Vol. 78, No. 24
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.24.14039-14042.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Neutralization Profiles of Newly Transmitted Human Immunodeficiency Virus Type 1 by Monoclonal Antibodies 2G12, 2F5, and 4E10

Saurabh Mehandru,1 Terri Wrin,2 Justin Galovich,2 Gabriela Stiegler,3 Brigitta Vcelar,3 Arlene Hurley,1 Christine Hogan,1 Sandhya Vasan,1 Hermann Katinger,3 Christos J. Petropoulos,2 and Martin Markowitz1*

Aaron Diamond AIDS Research Center, Rockefeller University, New York, New York,1 ViroLogic, Inc., South San Francisco, California,2 Polymun Scientific, Vienna, Austria3

Received 7 May 2004/ Accepted 6 August 2004

As the AIDS epidemic continues unabated, the development of a human immunodeficiency virus (HIV) vaccine is critical. Ideally, an effective vaccine should elicit cell-mediated and neutralizing humoral immune responses. We have determined the in vitro susceptibility profile of sexually transmitted viruses from 91 patients with acute and early HIV-1 infection to three monoclonal antibodies, 2G12, 2F5, and 4E10. Using a recombinant virus assay to measure neutralization, we found all transmitted viruses were neutralized by 4E10, 80% were neutralized by 2F5, and only 37% were neutralized by 2G12. We propose that the induction of 4E10-like antibodies should be a priority in designing immunogens to prevent HIV-1 infection.

* Corresponding author. Mailing address: Aaron Diamond AIDS Research Center, The Rockefeller University, 455 First Ave., 7th Floor, New York, NY 10016. Phone: (212) 448-5020. Fax: (212) 725-1126. E-mail: mmarkowitz{at}adarc.org.

Journal of Virology, December 2004, p. 14039-14042, Vol. 78, No. 24
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.24.14039-14042.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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