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Journal of Virology, December 2004, p. 13829-13838, Vol. 78, No. 24
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.24.13829-13838.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genome Assembly and Particle Maturation of the Birnavirus Infectious Pancreatic Necrosis Virus

Rodrigo A. Villanueva,* José L. Galaz, Juan A. Valdés, Matilde M. Jashés, and Ana María Sandino

Laboratorio de Virología, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile

Received 23 March 2004/ Accepted 11 August 2004

In this study, we have analyzed the morphogenesis of the birnavirus infectious pancreatic necrosis virus throughout the infective cycle in CHSE-214 cells by using a native agarose electrophoresis system. Two types of viral particles (designated A and B) were identified, isolated, and characterized both molecularly and biologically. Together, our results are consistent with a model of morphogenesis in which the genomic double-stranded RNA is immediately assembled, after synthesis, into a large (66-nm diameter) and uninfectious particle A, where the capsid is composed of both mature and immature viral polypeptides. Upon maturation, particles A yield particles B through the proteolytic cleavage of most of the remaining viral precursors within the capsid, the compaction of the particle (60-nm diameter), and the acquisition of infectivity. These studies will provide the foundation for further analyses of birnavirus particle assembly and RNA replication.

* Corresponding author. Present address: Unité Hépatite C, CNRS-UPR2511, IBL/Institut Pasteur de Lille. 1, rue du Professeur Calmette, B.P. 447, 59021 Lille Cedex, France. Phone: 333 2087 1092. Fax: 333 2087 1201. E-mail: rodrigo.villanueva{at}ibl.fr.

Journal of Virology, December 2004, p. 13829-13838, Vol. 78, No. 24
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.24.13829-13838.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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