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Journal of Virology, December 2004, p. 13376-13380, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13376-13380.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes

Lawrence Petherbridge,1 Andrew C. Brown,1 Susan J. Baigent,1 Ken Howes,1 Melanie A. Sacco,1,{dagger} Nikolaus Osterrieder,2 and Venugopal K. Nair1*

Viral Oncogenesis Group, Institute for Animal Health, Compton, Berkshire, United Kingdom,1 Department of Microbiology and Immunology, Cornell University, Ithaca, New York2

Received 25 May 2004/ Accepted 7 July 2004

Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that induces T-cell lymphomas in poultry. We report the construction of bacterial artificial chromosome (BAC) clones of the highly oncogenic RB-1B strain by inserting mini-F vector sequences into the US2 locus. MDV reconstituted from two BAC clones induced rapid-onset lymphomas similar to those induced by the wild-type virus. Virus reconstituted from another BAC clone that showed a 7.7-kbp deletion in the internal and terminal unique long repeat regions was nononcogenic, suggesting that the deleted region may be associated with oncogenicity. The generation of the oncogenic BAC clones of MDV is a significant step in unraveling the oncogenic determinants of this virus.

* Corresponding author. Mailing address: Viral Oncogenesis Group, Institute for Animal Health, High St., Compton, Berkshire RG20 7NN, United Kingdom. Phone: 44-1635-577356. Fax: 44-1635-577263. E-mail: venu.gopal{at}bbsrc.ac.uk.

{dagger} Present address: Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853.

Journal of Virology, December 2004, p. 13376-13380, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13376-13380.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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