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Journal of Virology, December 2004, p. 13370-13375, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13370-13375.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of Murine Gammaherpesvirus 68 Glycoprotein B

Filipa B. Lopes, Susanna Colaco, Janet S. May, and Philip G. Stevenson*

Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom

Received 26 May 2004/ Accepted 23 July 2004

Murine gammaherpesvirus 68 (MHV-68) glycoprotein B (gB) was identified in purified virions by immunoblotting, immunoprecipitation, and immunoelectron microscopy. It was synthesized as a 120-kDa precursor in infected cells and cleaved into 65-kDa and 55-kDa disulfide-linked subunits close to the time of virion release. The N-linked glycans on the cleaved, virion gB remained partially endoglycosidase H sensitive. The processing of MHV-68 gB therefore appears similar to that of Kaposi's sarcoma-associated herpesvirus gB and human cytomegalovirus gB.

* Corresponding author. Mailing address: Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom. Phone: 44-1223-336921. Fax: 44-1223-336926. E-mail: pgs27{at}mole.bio.cam.ac.uk.

Journal of Virology, December 2004, p. 13370-13375, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13370-13375.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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