This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Holterman, L. Articles by Havenga, M. Search for Related Content PubMed PubMed Citation Articles by Holterman, L. Articles by Havenga, M.

Novel Replication-Incompetent Vector Derived from Adenovirus Type 11 (Ad11) for Vaccination and Gene Therapy: Low Seroprevalence and Non-Cross-Reactivity with Ad5

Crucell Holland BV,¹ Gaubius Laboratory, TNO-PG,² Department of Surgery, Leiden University Medical Center, Leiden,⁴ Department of Human Retrovirology, Academic Medical Center, Amsterdam, The Netherlands³

Received 1 June 2004/ Accepted 26 June 2004

A novel plasmid-based adenovirus vector system that enables manufacturing of replication-incompetent (E1) adenovirus type 11 (Ad11)-based vectors is described. Ad11 vectors are produced on PER.C6/55K cells yielding high-titer vector batches after purification. Ad11 seroprevalence proves to be significantly lower than that of Ad5, and neutralizing antibody titers against Ad11 are low. Ad11 seroprevalence among human immunodeficiency virus-positive (HIV⁺) individuals is as low as that among HIV^– individuals, independent of the level of immune suppression. The low level of coinciding seroprevalence between Ad11 and Ad35 in addition to a lack of correlation between high neutralizing antibody titers towards either adenovirus strongly suggest that the limited humoral cross-reactive immunity between these two highly related B viruses appears not to preclude the use of both vectors in the same individual. Ad11 transduces primary cells including smooth muscle cells, synoviocytes, and dendritic cells and cardiovascular tissues with higher efficiency than Ad5. Ad11 and Ad35 appear to have a similar tropism as judged by green fluorescent protein expression levels determined by using a panel of cancer cell lines. In addition, Ad5 preimmunization did not significantly affect Ad11-mediated transduction in C57BL/6 mice. We therefore conclude that the Ad11-based vector represents a novel and useful candidate gene transfer vehicle for vaccination and gene therapy.

This article has been cited by other articles:

• Schoenly, K. A., Weiner, D. B. (2008). Human Immunodeficiency Virus Type 1 Vaccine Development: Recent Advances in the Cytotoxic T-Lymphocyte Platform "Spotty Business". J. Virol. 82: 3166-3180 [Full Text]  
• Fleischli, C., Sirena, D., Lesage, G., Havenga, M. J. E., Cattaneo, R., Greber, U. F., Hemmi, S. (2007). Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor. J. Gen. Virol. 88: 2925-2934 [Abstract] [Full Text]  
• Abbink, P., Lemckert, A. A. C., Ewald, B. A., Lynch, D. M., Denholtz, M., Smits, S., Holterman, L., Damen, I., Vogels, R., Thorner, A. R., O'Brien, K. L., Carville, A., Mansfield, K. G., Goudsmit, J., Havenga, M. J. E., Barouch, D. H. (2007). Comparative Seroprevalence and Immunogenicity of Six Rare Serotype Recombinant Adenovirus Vaccine Vectors from Subgroups B and D. J. Virol. 81: 4654-4663 [Abstract] [Full Text]  
• Koizumi, N., Yamaguchi, T., Kawabata, K., Sakurai, F., Sasaki, T., Watanabe, Y., Hayakawa, T., Mizuguchi, H. (2007). Fiber-Modified Adenovirus Vectors Decrease Liver Toxicity through Reduced IL-6 Production. J. Immunol. 178: 1767-1773 [Abstract] [Full Text]  
• Thorner, A. R., Lemckert, A. A. C., Goudsmit, J., Lynch, D. M., Ewald, B. A., Denholtz, M., Havenga, M. J. E., Barouch, D. H. (2006). Immunogenicity of Heterologous Recombinant Adenovirus Prime-Boost Vaccine Regimens Is Enhanced by Circumventing Vector Cross-Reactivity. J. Virol. 80: 12009-12016 [Abstract] [Full Text]  
• Thorner, A. R., Vogels, R., Kaspers, J., Weverling, G. J., Holterman, L., Lemckert, A. A. C., Dilraj, A., McNally, L. M., Jeena, P. M., Jepsen, S., Abbink, P., Nanda, A., Swanson, P. E., Bates, A. T., O'Brien, K. L., Havenga, M. J. E., Goudsmit, J., Barouch, D. H. (2006). Age dependence of adenovirus-specific neutralizing antibody titers in individuals from sub-saharan Africa.. J. Clin. Microbiol. 44: 3781-3783 [Abstract] [Full Text]  
• Lemckert, A. A. C., Grimbergen, J., Smits, S., Hartkoorn, E., Holterman, L., Berkhout, B., Barouch, D. H., Vogels, R., Quax, P., Goudsmit, J., Havenga, M. J. E. (2006). Generation of a novel replication-incompetent adenoviral vector derived from human adenovirus type 49: manufacture on PER.C6 cells, tropism and immunogenicity.. J. Gen. Virol. 87: 2891-2899 [Abstract] [Full Text]  
• Havenga, M., Vogels, R., Zuijdgeest, D., Radosevic, K., Mueller, S., Sieuwerts, M., Weichold, F., Damen, I., Kaspers, J., Lemckert, A., van Meerendonk, M., van der Vlugt, R., Holterman, L., Hone, D., Skeiky, Y., Mintardjo, R., Gillissen, G., Barouch, D., Sadoff, J., Goudsmit, J. (2006). Novel replication-incompetent adenoviral B-group vectors: high vector stability and yield in PER.C6 cells.. J. Gen. Virol. 87: 2135-2143 [Abstract] [Full Text]  
• Ruzsics, Z., Wagner, M., Osterlehner, A., Cook, J., Koszinowski, U., Burgert, H.-G. (2006). Transposon-assisted cloning and traceless mutagenesis of adenoviruses: development of a novel vector based on species d.. J. Virol. 80: 8100-8113 [Abstract] [Full Text]  
• Verhaagh, S., de Jong, E., Goudsmit, J., Lecollinet, S., Gillissen, G., de Vries, M., van Leuven, K., Que, I., Ouwehand, K., Mintardjo, R., Weverling, G. J., Radosevic, K., Richardson, J., Eloit, M., Lowik, C., Quax, P., Havenga, M. (2006). Human CD46-transgenic mice in studies involving replication-incompetent adenoviral type 35 vectors. J. Gen. Virol. 87: 255-265 [Abstract] [Full Text]  
• McConnell, M. J., Hanna, P. C., Imperiale, M. J. (2006). Cytokine Response and Survival of Mice Immunized with an Adenovirus Expressing Bacillus anthracis Protective Antigen Domain 4. Infect. Immun. 74: 1009-1015 [Abstract] [Full Text]  
• Ophorst, O. J. A. E., Radosevic, K., Havenga, M. J. E., Pau, M. G., Holterman, L., Berkhout, B., Goudsmit, J., Tsuji, M. (2006). Immunogenicity and Protection of a Recombinant Human Adenovirus Serotype 35-Based Malaria Vaccine against Plasmodium yoelii in Mice. Infect. Immun. 74: 313-320 [Abstract] [Full Text]  
• McDermott, A. B., O'Connor, D. H., Fuenger, S., Piaskowski, S., Martin, S., Loffredo, J., Reynolds, M., Reed, J., Furlott, J., Jacoby, T., Riek, C., Dodds, E., Krebs, K., Davies, M.-E., Schleif, W. A., Casimiro, D. R., Shiver, J. W., Watkins, D. I. (2005). Cytotoxic T-Lymphocyte Escape Does Not Always Explain the Transient Control of Simian Immunodeficiency Virus SIVmac239 Viremia in Adenovirus-Boosted and DNA-Primed Mamu-A*01-Positive Rhesus Macaques. J. Virol. 79: 15556-15566 [Abstract] [Full Text]  
• Nanda, A., Lynch, D. M., Goudsmit, J., Lemckert, A. A. C., Ewald, B. A., Sumida, S. M., Truitt, D. M., Abbink, P., Kishko, M. G., Gorgone, D. A., Lifton, M. A., Shen, L., Carville, A., Mansfield, K. G., Havenga, M. J. E., Barouch, D. H. (2005). Immunogenicity of Recombinant Fiber-Chimeric Adenovirus Serotype 35 Vector-Based Vaccines in Mice and Rhesus Monkeys. J. Virol. 79: 14161-14168 [Abstract] [Full Text]  
• Hensley, S. E., Giles-Davis, W., McCoy, K. C., Weninger, W., Ertl, H. C. J. (2005). Dendritic Cell Maturation, but Not CD8+ T Cell Induction, Is Dependent on Type I IFN Signaling during Vaccination with Adenovirus Vectors. J. Immunol. 175: 6032-6041 [Abstract] [Full Text]  
• Lemckert, A. A. C., Sumida, S. M., Holterman, L., Vogels, R., Truitt, D. M., Lynch, D. M., Nanda, A., Ewald, B. A., Gorgone, D. A., Lifton, M. A., Goudsmit, J., Havenga, M. J. E., Barouch, D. H. (2005). Immunogenicity of Heterologous Prime-Boost Regimens Involving Recombinant Adenovirus Serotype 11 (Ad11) and Ad35 Vaccine Vectors in the Presence of Anti-Ad5 Immunity. J. Virol. 79: 9694-9701 [Abstract] [Full Text]  
• Vellinga, J., Van der Heijdt, S., Hoeben, R. C. (2005). The adenovirus capsid: major progress in minor proteins. J. Gen. Virol. 86: 1581-1588 [Abstract] [Full Text]