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Journal of Virology, December 2004, p. 13197-13206, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13197-13206.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Expression and Characterization of a Soluble Form of Tomato Spotted Wilt Virus Glycoprotein GN

Anna E. Whitfield,1 Diane E. Ullman,2 and Thomas L. German1*

Departments of Entomology and Plant Pathology, University of Wisconsin, Madison, Wisconsin,1 Department of Entomology, University of California, Davis, California2

Received 27 April 2004/ Accepted 28 July 2004

Tomato spotted wilt virus (TSWV), a member of the Tospovirus genus within the Bunyaviridae, is an economically important plant pathogen with a worldwide distribution. TSWV is transmitted to plants via thrips (Thysanoptera: Thripidae), which transmit the virus in a persistent propagative manner. The envelope glycoproteins, GN and GC, are critical for the infection of thrips, but they are not required for the initial infection of plants. Thus, it is assumed that the envelope glycoproteins play important roles in the entry of TSWV into the insect midgut, the first site of infection. To directly test the hypothesis that GN plays a role in TSWV acquisition by thrips, we expressed and purified a soluble, recombinant form of the GN protein (GN-S). The expression of GN-S allowed us to examine the function of GN in the absence of other viral proteins. We detected specific binding to thrips midguts when purified GN-S was fed to thrips in an in vivo binding assay. The TSWV nucleocapsid protein and human cytomegalovirus glycoprotein B did not bind to thrips midguts, indicating that the GN-S-thrips midgut interaction is specific. TSWV acquisition inhibition assays revealed that thrips that were concomitantly fed purified TSWV and GN-S had reduced amounts of virus in their midguts compared to thrips that were fed TSWV only. Our findings that GN-S binds to larval thrips guts and decreases TSWV acquisition provide evidence that GN may serve as a viral ligand that mediates the attachment of TSWV to receptors displayed on the epithelial cells of the thrips midgut.

* Corresponding author. Mailing address: Department of Entomology, University of Wisconsin, 1630 Linden Dr., Madison, WI 53706. Phone: (608) 262-1696. Fax: (608) 262-3322. E-mail: tlg{at}entomology.wisc.edu.

Journal of Virology, December 2004, p. 13197-13206, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13197-13206.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ullman, D. E., Whitfield, A. E., German, T. L. (2005). Thrips and tospoviruses come of age: Mapping determinants of insect transmission. Proc. Natl. Acad. Sci. USA 102: 4931-4932 [Full Text]  


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