This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Provost, B. Articles by Drezen, J.-M. Search for Related Content PubMed PubMed Citation Articles by Provost, B. Articles by Drezen, J.-M.

 Previous Article  |  Next Article 

Journal of Virology, December 2004, p. 13090-13103, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13090-13103.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Bracoviruses Contain a Large Multigene Family Coding for Protein Tyrosine Phosphatases

Bertille Provost,¹ Paola Varricchio,² Eloisa Arana,³ Eric Espagne,^1, Patrizia Falabella,⁴ Elisabeth Huguet,¹ Raffaella La Scaleia,⁴ Laurence Cattolico,⁵ Marylène Poirié,¹ Carla Malva,² Julie A. Olszewski,³ Francesco Pennacchio,⁴ and Jean-Michel Drezen^1*

Institut de Recherche sur la Biologie de l'Insecte, UMR CNRS 6035, Faculté des Sciences et Techniques, Tours,¹ Genoscope, Centre National de Séquençage, Evry, France,⁵ Istituto di Genetica e Biofisica, Consiglio Nazionale della Richerche, Naples,² Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università della Basilicata-Macchia Romana, Potenza, Italy,⁴ Department of Biological Sciences, Imperial College, London, United Kingdom³

Received 21 April 2004/ Accepted 19 July 2004

The relationship between parasitic wasps and bracoviruses constitutes one of the few known mutualisms between viruses and eukaryotes. The virions produced in the wasp ovaries are injected into host lepidopteran larvae, where virus genes are expressed, allowing successful development of the parasite by inducing host immune suppression and developmental arrest. Bracovirus-bearing wasps have a common phylogenetic origin, and contemporary bracoviruses are hypothesized to have been inherited by chromosomal transmission from a virus that originally integrated into the genome of the common ancestor wasp living 73.7 ± 10 million years ago. However, so far no conserved genes have been described among different braconid wasp subfamilies. Here we show that a gene family is present in bracoviruses of different braconid wasp subfamilies (Cotesia congregata, Microgastrinae, and Toxoneuron nigriceps, Cardiochilinae) which likely corresponds to an ancient component of the bracovirus genome that might have been present in the ancestral virus. The genes encode proteins belonging to the protein tyrosine phosphatase family, known to play a key role in the control of signal transduction pathways. Bracovirus protein tyrosine phosphatase genes were shown to be expressed in different tissues of parasitized hosts, and two protein tyrosine phosphatases were produced with recombinant baculoviruses and tested for their biochemical activity. One protein tyrosine phosphatase is a functional phosphatase. These results strengthen the hypothesis that protein tyrosine phosphatases are involved in virally induced alterations of host physiology during parasitism.

---

* Corresponding author. Mailing address: Institut de Recherche sur la Biologie de l'Insecte, UMR CNRS 6035, Faculté des Sciences et Techniques, Parc Grandmont, 37200 Tours, France. Phone: 33 (0)2 47 36 73 57. Fax: 33 (0)2 47 36 69 66. E-mail: drezen{at}univ-tours.fr.

Present address: Institut de Génétique et Microbiologie, UMR CNRS 8621, Université Paris Sud, Orsay Cedex, France.

---

Journal of Virology, December 2004, p. 13090-13103, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13090-13103.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Rasoolizadeh, A., Beliveau, C., Stewart, D., Cloutier, C., Cusson, M. (2009). Tranosema rostrale ichnovirus repeat element genes display distinct transcriptional patterns in caterpillar and wasp hosts. J. Gen. Virol. 90: 1505-1514 [Abstract] [Full Text]  
• Bezier, A., Annaheim, M., Herbiniere, J., Wetterwald, C., Gyapay, G., Bernard-Samain, S., Wincker, P., Roditi, I., Heller, M., Belghazi, M., Pfister-Wilhem, R., Periquet, G., Dupuy, C., Huguet, E., Volkoff, A.-N., Lanzrein, B., Drezen, J.-M. (2009). Polydnaviruses of Braconid Wasps Derive from an Ancestral Nudivirus. Science 323: 926-930 [Abstract] [Full Text]  
• Chen, Y.-F., Shi, M., Huang, F., Chen, X.-x. (2007). Characterization of two genes of Cotesia vestalis polydnavirus and their expression patterns in the host Plutella xylostella. J. Gen. Virol. 88: 3317-3322 [Abstract] [Full Text]  
• Pruijssers, A. J., Strand, M. R. (2007). PTP-H2 and PTP-H3 from Microplitis demolitor Bracovirus Localize to Focal Adhesions and Are Antiphagocytic in Insect Immune Cells. J. Virol. 81: 1209-1219 [Abstract] [Full Text]  
• Falabella, P., Varricchio, P., Provost, B., Espagne, E., Ferrarese, R., Grimaldi, A., de Eguileor, M., Fimiani, G., Ursini, M. V., Malva, C., Drezen, J.-M., Pennacchio, F. (2007). Characterization of the I{kappa}B-like gene family in polydnaviruses associated with wasps belonging to different Braconid subfamilies. J. Gen. Virol. 88: 92-104 [Abstract] [Full Text]  
• Gundersen-Rindal, D. E., Pedroni, M. J. (2006). Characterization and transcriptional analysis of protein tyrosine phosphatase genes and an ankyrin repeat gene of the parasitoid Glyptapanteles indiensis polydnavirus in the parasitized host. J. Gen. Virol. 87: 311-322 [Abstract] [Full Text]  
• Espagne, E., Douris, V., Lalmanach, G., Provost, B., Cattolico, L., Lesobre, J., Kurata, S., Iatrou, K., Drezen, J.-M., Huguet, E. (2005). A Virus Essential for Insect Host-Parasite Interactions Encodes Cystatins. J. Virol. 79: 9765-9776 [Abstract] [Full Text]