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Journal of Virology, November 2004, p. 12717-12721, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12717-12721.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Study of Sequence Variation of Dengue Type 3 Virus in Naturally Infected Mosquitoes and Human Hosts: Implications for Transmission and Evolution

Su-Ru Lin,1 Szu-Chia Hsieh,1 Yi-Yuan Yueh,2 Ting-Hsiang Lin,2 Day-Yu Chao,3 Wei-June Chen,4 Chwan-Chuen King,3 and Wei-Kung Wang1,5*

Institute of Microbiology,1 Department of Internal Medicine,5 College of Medicine, and Institute of Epidemiology, College of Public Health, National Taiwan University,3 Division of Laboratory Research and Development, Center for Disease Control, Department of Health, Taipei,2 Department of Public Health and Parasitology, Chang-Guan University, Tao-Yuan, Taiwan4

Received 1 May 2004/ Accepted 1 July 2004

Dengue virus is an arbovirus that replicates alternately in the mosquito vector and human host. We investigated sequences of dengue type 3 virus in naturally infected Aedes aegypti mosquitoes and in eight patients from the same outbreak and reported that the extent of sequence variation seen with the mosquitoes was generally lower than that seen with the patients (mean diversity, 0.21 versus 0.38% and 0.09 versus 0.23% for the envelope [E] and capsid [C] genes, respectively). This was further verified with five experimentally infected mosquitoes (mean diversity, 0.09 and 0.10% for the E and C genes, respectively). Examination of the quasispecies structures of the E sequences of the mosquitoes and of the patients revealed that the sequences of the major variants were the same, suggesting that the major variant was transmitted. These findings support our hypothesis that mosquitoes contribute to the evolutionary conservation of dengue virus by maintaining a more homogenous viral population and a dominant variant during transmission.

* Corresponding author. Mailing address: Institute of Microbiology, College of Medicine, National Taiwan University, No. 1 Sec. 1 Jen-Ai Rd., Taipei, Taiwan. Phone: 886-2-2312-3456, ext. 8286. Fax: 886-2-2391-5293. E-mail: wwang60{at}yahoo.com.

Journal of Virology, November 2004, p. 12717-12721, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12717-12721.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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