This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Op De Beeck, A.
Right arrow Articles by Dubuisson, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Op De Beeck, A.
Right arrow Articles by Dubuisson, J.

 Previous Article  |  Next Article 

Journal of Virology, November 2004, p. 12591-12602, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12591-12602.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Transmembrane Domains of the prM and E Proteins of Yellow Fever Virus Are Endoplasmic Reticulum Localization Signals

Anne Op De Beeck,1,{dagger} Yves Rouillé,1,{dagger} Mélanie Caron,1 Sandrine Duvet,2 and Jean Dubuisson1*

CNRS-UPR2511, Institut de Biologie de Lille & Institut Pasteur de Lille, Lille,1 CNRS-UMR8576, Université des Sciences et Technologies, Villeneuve d'Ascq France2

Received 29 September 2003/ Accepted 3 June 2004

The immature flavivirus particle contains two envelope proteins, prM and E, that are associated as a heterodimer. Virion morphogenesis of the flaviviruses occurs in association with endoplasmic reticulum (ER) membranes, suggesting that there should be accumulation of the virion components in this compartment. This also implies that ER localization signals must be present in the flavivirus envelope proteins. In this work, we looked for potential subcellular localization signals in the yellow fever virus envelope proteins. Confocal immunofluorescence analysis of the subcellular localization of the E protein in yellow fever virus-infected cells indicated that this protein accumulates in the ER. Similar results were obtained with cells expressing only prM and E. Chimeric proteins containing the ectodomain of CD4 or CD8 fused to the transmembrane domains of prM or E were constructed, and their subcellular localization was studied by confocal immunofluorescence and by analyzing the maturation of their associated glycans. Although a small fraction was detected in the ER-to-Golgi intermediate and Golgi compartments, these chimeric proteins were located mainly in the ER. The C termini of prM and E form two antiparallel transmembrane {alpha}-helices. Interestingly, the first transmembrane passage contains enough information for ER localization. Taken altogether, these data indicate that, besides their role as membrane anchors, the transmembrane domains of yellow fever virus envelope proteins are ER retention signals. In addition, our data show that the mechanisms of ER retention of the flavivirus and hepacivirus envelope proteins are different.

* Corresponding author. Mailing address: Unité Hépatite C, CNRS-UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille cedex, France. Phone: (33) 3 20 87 11 60. Fax: (33) 3 20 87 12 01. E-mail: jean.dubuisson{at}ibl.fr.

{dagger} These authors contributed equally to this study.

Journal of Virology, November 2004, p. 12591-12602, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12591-12602.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ciczora, Y., Callens, N., Seron, K., Rouille, Y., Dubuisson, J. (2010). Identification of a dominant endoplasmic reticulum-retention signal in yellow fever virus pre-membrane protein. J. Gen. Virol. 91: 404-414 [Abstract] [Full Text]  
  • Orlinger, K. K., Hoenninger, V. M., Kofler, R. M., Mandl, C. W. (2006). Construction and Mutagenesis of an Artificial Bicistronic Tick-Borne Encephalitis Virus Genome Reveals an Essential Function of the Second Transmembrane Region of Protein E in Flavivirus Assembly. J. Virol. 80: 12197-12208 [Abstract] [Full Text]  
  • Meertens, L., Bertaux, C., Dragic, T. (2006). Hepatitis C Virus Entry Requires a Critical Postinternalization Step and Delivery to Early Endosomes via Clathrin-Coated Vesicles. J. Virol. 80: 11571-11578 [Abstract] [Full Text]  
  • McElroy, K. L., Tsetsarkin, K. A., Vanlandingham, D. L., Higgs, S. (2006). Role of the yellow fever virus structural protein genes in viral dissemination from the Aedes aegypti mosquito midgut.. J. Gen. Virol. 87: 2993-3001 [Abstract] [Full Text]  
  • Vainauskas, S., Menon, A. K. (2005). Endoplasmic Reticulum Localization of Gaa1 and PIG-T, Subunits of the Glycosylphosphatidylinositol Transamidase Complex. J. Biol. Chem. 280: 16402-16409 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»