This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brussel, A.
Right arrow Articles by Sonigo, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brussel, A.
Right arrow Articles by Sonigo, P.

 Previous Article  |  Next Article 

Journal of Virology, October 2004, p. 11263-11271, Vol. 78, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.20.11263-11271.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evidence for Gene Expression by Unintegrated Human Immunodeficiency Virus Type 1 DNA Species

Audrey Brussel{dagger} and Pierre Sonigo*

Département des Maladies Infectieuses, Institut Cochin, INSERM U567, CNRS UMR 8104, Université René Descartes, Paris, France

Received 6 April 2004/ Accepted 2 June 2004

The integrated form of human immunodeficiency virus type 1 (HIV-1) DNA is classically considered to be the sole template for viral gene expression. However, several studies have suggested that unintegrated viral DNA species could also support transcription. To determine the contribution of the different species of HIV-1 DNA to viral expression, we first monitored intracellular levels of various HIV-1 DNA and RNA species in a single-round infection assay. We observed that, in comparison to the precocity of HIV-1 DNA synthesis, viral expression was delayed, suggesting that only the HIV-1 DNA species that persist for a sufficient period of time would be transcribed efficiently. We next evaluated the transcriptional activity of the circular forms of HIV-1 DNA bearing two long terminal repeats, since these episomes were reported to exhibit an intrinsic molecular stability. Our results support the notion that these circular species of HIV-1 DNA are naturally transcribed during HIV-1 infection, thereby participating in virus replication.

* Corresponding author. Mailing address: Département des Maladies Infectieuses, Institut Cochin, INSERM U567, CNRS UMR 8104, Université René Descartes, 22 rue Méchain, 75014 Paris, France. Phone: 33 1 40 51 64 13. Fax: 33 1 40 51 64 07. E-mail: sonigo{at}cochin.inserm.fr.

{dagger} Present address: Unité de Physiopathologie des Infections Lentivirales, Institut Pasteur, 75015 Paris, France.

Journal of Virology, October 2004, p. 11263-11271, Vol. 78, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.20.11263-11271.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Iyer, S. R., Yu, D., Biancotto, A., Margolis, L. B., Wu, Y. (2009). Measurement of Human Immunodeficiency Virus Type 1 Preintegration Transcription by Using Rev-Dependent Rev-CEM Cells Reveals a Sizable Transcribing DNA Population Comparable to That from Proviral Templates. J. Virol. 83: 8662-8673 [Abstract] [Full Text]  
  • Nishimura, Y., Sadjadpour, R., Mattapallil, J. J., Igarashi, T., Lee, W., Buckler-White, A., Roederer, M., Chun, T.-W., Martin, M. A. (2009). High frequencies of resting CD4+ T cells containing integrated viral DNA are found in rhesus macaques during acute lentivirus infections. Proc. Natl. Acad. Sci. USA 106: 8015-8020 [Abstract] [Full Text]  
  • Noursadeghi, M., Tsang, J., Miller, R. F., Straschewski, S., Kellam, P., Chain, B. M., Katz, D. R. (2009). Genome-Wide Innate Immune Responses in HIV-1-Infected Macrophages Are Preserved Despite Attenuation of the NF-{kappa}B Activation Pathway. J. Immunol. 182: 319-328 [Abstract] [Full Text]  
  • Marini, A., Harper, J. M., Romerio, F. (2008). An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency. J. Immunol. 181: 7713-7720 [Abstract] [Full Text]  
  • Poon, B., Chang, M. A., Chen, I. S. Y. (2007). Vpr Is Required for Efficient Nef Expression from Unintegrated Human Immunodeficiency Virus Type 1 DNA. J. Virol. 81: 10515-10523 [Abstract] [Full Text]  
  • Sundstrom, J. B., Ellis, J. E., Hair, G. A., Kirshenbaum, A. S., Metcalfe, D. D., Yi, H., Cardona, A. C., Lindsay, M. K., Ansari, A. A. (2007). Human tissue mast cells are an inducible reservoir of persistent HIV infection. Blood 109: 5293-5300 [Abstract] [Full Text]  
  • Thomas, J. A., Ott, D. E., Gorelick, R. J. (2007). Efficiency of Human Immunodeficiency Virus Type 1 Postentry Infection Processes: Evidence against Disproportionate Numbers of Defective Virions. J. Virol. 81: 4367-4370 [Abstract] [Full Text]  
  • Cara, A., Klotman, M. E. (2006). Retroviral E-DNA: persistence and gene expression in nondividing immune cells. J. Leukoc. Biol. 80: 1013-1017 [Abstract] [Full Text]  
  • Bona, R., Andreotti, M., Buffa, V., Leone, P., Galluzzo, C. M., Amici, R., Palmisano, L., Mancini, M. G., Michelini, Z., Di Santo, R., Costi, R., Roux, A., Pommier, Y., Marchand, C., Vella, S., Cara, A. (2006). Development of a human immunodeficiency virus vector-based, single-cycle assay for evaluation of anti-integrase compounds.. Antimicrob. Agents Chemother. 50: 3407-3417 [Abstract] [Full Text]  
  • Arhel, N., Munier, S., Souque, P., Mollier, K., Charneau, P. (2006). Nuclear import defect of human immunodeficiency virus type 1 DNA flap mutants is not dependent on the viral strain or target cell type.. J. Virol. 80: 10262-10269 [Abstract] [Full Text]  
  • Miles, L. R., Agresta, B. E., Khan, M. B., Tang, S., Levin, J. G., Powell, M. D. (2005). Effect of Polypurine Tract (PPT) Mutations on Human Immunodeficiency Virus Type 1 Replication: a Virus with a Completely Randomized PPT Retains Low Infectivity. J. Virol. 79: 6859-6867 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»