This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jaimes, M. C.
Right arrow Articles by Greenberg, H. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jaimes, M. C.
Right arrow Articles by Greenberg, H. B.

 Previous Article  |  Next Article 

Journal of Virology, October 2004, p. 10967-10976, Vol. 78, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.20.10967-10976.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Maturation and Trafficking Markers on Rotavirus-Specific B Cells during Acute Infection and Convalescence in Children

María C. Jaimes,1,{dagger} Olga L. Rojas,2,{dagger} Eric J. Kunkel,3 Nicole H. Lazarus,3 Dulce Soler,4 Eugene C. Butcher,3 Dorsey Bass,5 Juana Angel,2 Manuel A. Franco,2,{dagger} and Harry B. Greenberg1*,{dagger}

Departments of Medicine, Microbiology, and Immunology,1 Laboratory of Immunology and Vascular Biology, Department of Pathology,3 Division of Pediatric Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, Stanford University School of Medicine, and Veterans Administration Palo Alto Health Care System, Stanford, California,5 Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá, Colombia,2 Inflammation Department, Millennium Pharmaceuticals, Cambridge, Massachusetts4

Received 19 April 2004/ Accepted 27 May 2004

We have previously studied B cells, from people and mice, that express rotavirus-specific surface immunoglobulin (RV-sIg) by flow cytometry with recombinant virus-like particles that contain green fluorescent protein. In the present study we characterized circulating B cells with RV-sIg in children with acute and convalescent infection. During acute infection, circulating RV-sIgD– B cells are predominantly large, CD38high, CD27high, CD138+/–, CCR6–, {alpha}4ß7+, CCR9+, CCR10+, cutaneous lymphocyte antigen-negative (CLA–), L-selectinint/–, and sIgM+, sIgG–, sIgA+/– lymphocytes. This phenotype likely corresponds to gut-targeted plasma cells and plasmablasts. During convalescence the phenotype switches to small and large lymphocytes, CD38int/–, CD27int/–, CCR6+, {alpha}4ß7+/–, CCR9+/– and CCR10–, most likely representing RV-specific memory B cells with both gut and systemic trafficking profiles. Of note, during acute RV infection both total and RV-specific murine IgM and IgA antibody-secreting cells migrate efficiently to CCL28 (the CCR10 ligand) and to a lesser extent to CCL25 (the CCR9 ligand). Our results show that CCR10 and CCR9 can be expressed on IgM as well as IgA antibody-secreting cells in response to acute intestinal infection, likely helping target these cells to the gut. However, these intestinal infection-induced plasmablasts lack the CLA homing receptor for skin, consistent with mechanisms of differential CCR10 participation in skin T versus intestinal plasma cell homing. Interestingly, RV memory cells generally lack CCR9 and CCR10 and instead express CCR6, which may enable recruitment to diverse epithelial sites of inflammation.

* Corresponding author. Mailing address: V.A. Palo Alto Health Care System, 3801 Miranda Ave., MC154C, Palo Alto, CA 94304. Phone: (650) 493-5000, ext. 63121. Fax: (650) 852-3259. E-mail: harry.greenberg{at}stanford.edu.

{dagger} M.C.J. and O.L.R. contributed equally to the work presented, and M.A.F. and H.B.G. contributed equally as senior authors.

Journal of Virology, October 2004, p. 10967-10976, Vol. 78, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.20.10967-10976.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mei, H. E., Yoshida, T., Sime, W., Hiepe, F., Thiele, K., Manz, R. A., Radbruch, A., Dorner, T. (2009). Blood-borne human plasma cells in steady state are derived from mucosal immune responses. Blood 113: 2461-2469 [Abstract] [Full Text]  
  • Doria-Rose, N. A., Klein, R. M., Manion, M. M., O'Dell, S., Phogat, A., Chakrabarti, B., Hallahan, C. W., Migueles, S. A., Wrammert, J., Ahmed, R., Nason, M., Wyatt, R. T., Mascola, J. R., Connors, M. (2009). Frequency and Phenotype of Human Immunodeficiency Virus Envelope-Specific B Cells from Patients with Broadly Cross-Neutralizing Antibodies. J. Virol. 83: 188-199 [Abstract] [Full Text]  
  • Gonzalez-Garcia, I., Rodriguez-Bayona, B., Mora-Lopez, F., Campos-Caro, A., Brieva, J. A. (2008). Increased survival is a selective feature of human circulating antigen-induced plasma cells synthesizing high-affinity antibodies. Blood 111: 741-749 [Abstract] [Full Text]  
  • Di Martino, C., Basset, C., Ogier, A., Charpilienne, A., Poncet, D., Kohli, E. (2007). Distribution and phenotype of rotavirus-specific B cells induced during the antigen-driven primary response to 2/6 virus-like particles administered by the intrarectal and the intranasal routes. J. Leukoc. Biol. 82: 821-828 [Abstract] [Full Text]  
  • Joshi, A. D., Hegde, G. V., Dickinson, J. D., Mittal, A. K., Lynch, J. C., Eudy, J. D., Armitage, J. O., Bierman, P. J., Bociek, R. G., Devetten, M. P., Vose, J. M., Joshi, S. S. (2007). ATM, CTLA4, MNDA, and HEM1 in High versus Low CD38 Expressing B-Cell Chronic Lymphocytic Leukemia. Clin. Cancer Res. 13: 5295-5304 [Abstract] [Full Text]  
  • Wang, Y., Dennehy, P. H., Keyserling, H. L., Tang, K., Gentsch, J. R., Glass, R. I., Jiang, B. (2007). Rotavirus Infection Alters Peripheral T-Cell Homeostasis in Children with Acute Diarrhea. J. Virol. 81: 3904-3912 [Abstract] [Full Text]  
  • Fecteau, J. F., Cote, G., Neron, S. (2006). A New Memory CD27-IgG+ B Cell Population in Peripheral Blood Expressing VH Genes with Low Frequency of Somatic Mutation. J. Immunol. 177: 3728-3736 [Abstract] [Full Text]  
  • Feng, N., Jaimes, M. C., Lazarus, N. H., Monak, D., Zhang, C., Butcher, E. C., Greenberg, H. B. (2006). Redundant Role of Chemokines CCL25/TECK and CCL28/MEC in IgA+ Plasmablast Recruitment to the Intestinal Lamina Propria After Rotavirus Infection. J. Immunol. 176: 5749-5759 [Abstract] [Full Text]  
  • Gonzalez-Garcia, I., Ocana, E., Jimenez-Gomez, G., Campos-Caro, A., Brieva, J. A. (2006). Immunization-Induced Perturbation of Human Blood Plasma Cell Pool: Progressive Maturation, IL-6 Responsiveness, and High PRDI-BF1/BLIMP1 Expression Are Critical Distinctions between Antigen-Specific and Nonspecific Plasma Cells. J. Immunol. 176: 4042-4050 [Abstract] [Full Text]  
  • Parez, N., Fourgeux, C., Mohamed, A., Dubuquoy, C., Pillot, M., Dehee, A., Charpilienne, A., Poncet, D., Schwartz-Cornil, I., Garbarg-Chenon, A. (2006). Rectal Immunization with Rotavirus Virus-Like Particles Induces Systemic and Mucosal Humoral Immune Responses and Protects Mice against Rotavirus Infection. J. Virol. 80: 1752-1761 [Abstract] [Full Text]  
  • Narvaez, C. F., Angel, J., Franco, M. A. (2005). Interaction of Rotavirus with Human Myeloid Dendritic Cells. J. Virol. 79: 14526-14535 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»