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Journal of Virology, January 2004, p. 892-898, Vol. 78, No. 2
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.2.892-898.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Reovirus of the Fungus Cryphonectria parasitica That Is Infectious as Particles and Related to the Coltivirus Genus of Animal Pathogens

Bradley I. Hillman,1* S. Supyani,2 Hideki Kondo,2 and Nobuhiro Suzuki2*

Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901-8520,1 Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan2

Received 23 June 2003/ Accepted 29 September 2003

RNA viruses of filamentous fungi fall into two broad categories, those that contain double-stranded RNA (dsRNA) genomes in rigid particles and those that are more closely related to positive-sense, single-stranded RNA viruses with dsRNA replicative intermediates found within lipid vesicles. Effective infectivity systems have been described for the latter, using RNA transcripts, but not for the former. We report the characterization of a reovirus from Cryphonectria parasitica, the filamentous fungus that causes chestnut blight disease. The virus substantially reduces the virulence of the fungus and results in dramatically altered colony morphology, as well as changes in other associated fungal traits, relative to the virus-free isogenic strain. Virus particles from infected mycelium contained 11 segments of dsRNA and showed characteristics typical of the family Reoviridae. Sequences of the largest three segments revealed that the virus is closely related to the Coltivirus genus of animal pathogens, which includes the human pathogen Colorado tick fever virus. The introduction of purified virus particles into protoplasts from virus-free isolates of the fungus resulted in a newly infected mycelium with the same morphology and virus composition as the original virus-infected isolate. This represents the completion of Koch's postulates for a true dsRNA virus from a filamentous fungus and the description of a definitive fungal member of the family Reoviridae.

* Corresponding author. Mailing address for B. I. Hillman: Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901-8520. Phone: (732) 932-9375, ext. 334. Fax: (732) 932-9377. E-mail: hillman{at}aesop.rutgers.edu. Mailing address for N. Suzuki: Research Institute for Bioresources, Okayama University, 2-20-1 Chu-ou, Kurashiki, Okayama 710-0046, Japan. Phone: 81-086-434-1230. Fax: 81-086-434-1232. E-mail: nsuzuki{at}rib.okayama-u.ac.jp.

Journal of Virology, January 2004, p. 892-898, Vol. 78, No. 2
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.2.892-898.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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