This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, H.
Right arrow Articles by Roossinck, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, H.
Right arrow Articles by Roossinck, M. J.

 Previous Article  |  Next Article 

Journal of Virology, October 2004, p. 10582-10587, Vol. 78, No. 19
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.19.10582-10587.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genetic Bottlenecks Reduce Population Variation in an Experimental RNA Virus Population

Hongye Li{dagger} and Marilyn J. Roossinck*

Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma

Received 15 March 2004/ Accepted 30 April 2004

Genetic bottlenecks are stochastic events that limit genetic variation in a population and result in founding populations that can lead to genetic drift. Evidence of past genetic bottlenecks in numerous biological systems, from mammals to viruses, has been described. In this study, we used an artificial population of Cucumber mosaic virus consisting of 12 restriction enzyme marker-bearing mutants. This population was inoculated onto young leaves of tobacco plants and monitored throughout the course of systemic infection. We show here that the genetic variation in a defined population of an RNA virus is significantly, stochastically, and reproducibly reduced during the systemic infection process, providing clear evidence of a genetic bottleneck.

* Corresponding author. Mailing address: The Samuel Roberts Noble Foundation, P.O. Box 2180, Ardmore, OK 73402. Phone: (580) 224-6600. Fax: (580) 224-6692. E-mail: mroossinck{at}noble.org.

{dagger} Present address: Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, People's Republic of China.

Journal of Virology, October 2004, p. 10582-10587, Vol. 78, No. 19
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.19.10582-10587.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gonzalez-Jara, P., Fraile, A., Canto, T., Garcia-Arenal, F. (2009). The Multiplicity of Infection of a Plant Virus Varies during Colonization of Its Eukaryotic Host. J. Virol. 83: 7487-7494 [Abstract] [Full Text]  
  • Escarmis, C., Perales, C., Domingo, E. (2009). Biological Effect of Muller's Ratchet: Distant Capsid Site Can Affect Picornavirus Protein Processing. J. Virol. 83: 6748-6756 [Abstract] [Full Text]  
  • Sanjuan, R., Agudelo-Romero, P., Elena, S. F. (2009). Upper-limit mutation rate estimation for a plant RNA virus. Biol Lett 5: 394-396 [Abstract] [Full Text]  
  • Hofmann, C., Niehl, A., Sambade, A., Steinmetz, A., Heinlein, M. (2009). Inhibition of Tobacco Mosaic Virus Movement by Expression of an Actin-Binding Protein. Plant Physiol. 149: 1810-1823 [Abstract] [Full Text]  
  • Betancourt, M., Fereres, A., Fraile, A., Garcia-Arenal, F. (2008). Estimation of the Effective Number of Founders That Initiate an Infection after Aphid Transmission of a Multipartite Plant Virus. J. Virol. 82: 12416-12421 [Abstract] [Full Text]  
  • Sanz-Ramos, M., Diaz-San Segundo, F., Escarmis, C., Domingo, E., Sevilla, N. (2008). Hidden Virulence Determinants in a Viral Quasispecies In Vivo. J. Virol. 82: 10465-10476 [Abstract] [Full Text]  
  • Simmons, H. E., Holmes, E. C., Stephenson, A. G. (2008). Rapid evolutionary dynamics of zucchini yellow mosaic virus. J. Gen. Virol. 89: 1081-1085 [Abstract] [Full Text]  
  • Turturo, C., Friscina, A., Gaubert, S., Jacquemond, M., Thompson, J. R., Tepfer, M. (2008). Evaluation of potential risks associated with recombination in transgenic plants expressing viral sequences. J. Gen. Virol. 89: 327-335 [Abstract] [Full Text]  
  • Moury, B., Fabre, F., Senoussi, R. (2007). Estimation of the number of virus particles transmitted by an insect vector. Proc. Natl. Acad. Sci. USA 104: 17891-17896 [Abstract] [Full Text]  
  • Pita, J. S., de Miranda, J. R., Schneider, W. L., Roossinck, M. J. (2007). Environment Determines Fidelity for an RNA Virus Replicase. J. Virol. 81: 9072-9077 [Abstract] [Full Text]  
  • Ge, L., Zhang, J., Zhou, X., Li, H. (2007). Genetic Structure and Population Variability of Tomato Yellow Leaf Curl China Virus. J. Virol. 81: 5902-5907 [Abstract] [Full Text]  
  • de la Iglesia, F., Elena, S. F. (2007). Fitness Declines in Tobacco Etch Virus upon Serial Bottleneck Transfers. J. Virol. 81: 4941-4947 [Abstract] [Full Text]  
  • Voth, P. D., Mairura, L., Lockhart, B. E., May, G. (2006). Phylogeography of Ustilago maydis virus H1 in the USA and Mexico.. J. Gen. Virol. 87: 3433-3441 [Abstract] [Full Text]  
  • Ali, A., Li, H., Schneider, W. L., Sherman, D. J., Gray, S., Smith, D., Roossinck, M. J. (2006). Analysis of Genetic Bottlenecks during Horizontal Transmission of Cucumber Mosaic Virus.. J. Virol. 80: 8345-8350 [Abstract] [Full Text]  
  • Thompson, J. R., Doun, S., Perry, K. L. (2006). Compensatory Capsid Protein Mutations in Cucumber Mosaic Virus Confer Systemic Infectivity in Squash (Cucurbita pepo).. J. Virol. 80: 7740-7743 [Abstract] [Full Text]  
  • Seemanpillai, M., Elamawi, R., Ritzenthaler, C., Heinlein, M. (2006). Challenging the role of microtubules in tobacco mosaic virus movement by drug treatments is disputable.. J. Virol. 80: 6712-6715 [Abstract] [Full Text]  
  • Pfeiffer, J. K., Kirkegaard, K. (2006). Bottleneck-mediated quasispecies restriction during spread of an RNA virus from inoculation site to brain. Proc. Natl. Acad. Sci. USA 103: 5520-5525 [Abstract] [Full Text]  
  • Jridi, C., Martin, J.-F., Marie-Jeanne, V., Labonne, G., Blanc, S. (2006). Distinct Viral Populations Differentiate and Evolve Independently in a Single Perennial Host Plant. J. Virol. 80: 2349-2357 [Abstract] [Full Text]  
  • Schirmer, A., Link, D., Cognat, V., Moury, B., Beuve, M., Meunier, A., Bragard, C., Gilmer, D., Lemaire, O. (2005). Phylogenetic analysis of isolates of Beet necrotic yellow vein virus collected worldwide. J. Gen. Virol. 86: 2897-2911 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»