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Journal of Virology, October 2004, p. 10276-10281, Vol. 78, No. 19
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.19.10276-10281.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Short Interfering RNA-Mediated Inhibition of Herpes Simplex Virus Type 1 Gene Expression and Function during Infection of Human Keratinocytes

Prakash K. Bhuyan,1* Katalin Karikò,2 John Capodici,1 John Lubinski,1 Lauren M. Hook,1 Harvey M. Friedman,1 and Drew Weissman1

Department of Medicine, Division of Infectious Diseases,1 Department of Neurosurgery, University of Pennsylvania, Philadelphia, Pennsylvania2

Received 10 March 2004/ Accepted 6 May 2004

RNA interference (RNAi) is an antiviral mechanism that is activated when double-stranded RNA is cleaved into fragments, called short interfering RNA (siRNA), that prime an inducible gene silencing enzyme complex. We applied RNAi against a herpes simplex virus type 1 (HSV-1) gene, glycoprotein E, which mediates cell-to-cell spread and immune evasion. In an in vitro model of infection, human keratinocytes were transfected with siRNA specific for glycoprotein E and then infected with wild-type HSV-1. RNAi-mediated gene silencing reproduced the small plaque phenotype of a gE-deletion mutant virus. The specificity of gene targeting was demonstrated by flow cytometry and Northern blot analyses. Exogenous siRNA can suppress HSV-1 glycoprotein E expression and function during active infection in vitro through RNAi. This work establishes RNAi as a genetic tool for the study of HSV and provides a foundation for development of RNAi as a novel antiviral therapy.

* Corresponding author. Mailing address: Division of Infectious Diseases, 3610 Hamilton Walk, Philadelphia, PA 19104. Phone: (215) 662-3185. Fax: (215) 349-5111. E-mail: bhuyan{at}prodigy.net.

Journal of Virology, October 2004, p. 10276-10281, Vol. 78, No. 19
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.19.10276-10281.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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