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Journal of Virology, September 2004, p. 9842-9853, Vol. 78, No. 18
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.18.9842-9853.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Consecutive Priming-Boosting Vaccination of Mice with Simian Immunodeficiency Virus (SIV) gag/pol DNA and Recombinant Vaccinia Virus Strain DIs Elicits Effective Anti-SIV Immunity

Kenji Someya,1,2 Ke-Qin Xin,2 Kazuhiro Matsuo,1 Kenji Okuda,2 Naoki Yamamoto,1 and Mitsuo Honda1*

AIDS Research Center, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo,1 Department of Bacteriology, Yokohama City University, School of Medicine, Fukuura, Kanazawa-ku, Yokohama, Japan2

Received 8 August 2003/ Accepted 18 May 2004

To evaluate immunity induced by a novel DNA prime-boost regimen, we constructed a DNA plasmid encoding the gag and pol genes from simian immunodeficiency virus (SIV) (SIVgag/pol DNA), in addition to a replication-deficient vaccinia virus strain DIs recombinant expressing SIV gag and pol genes (rDIsSIVgag/pol). In mice, priming with SIVgag/pol DNA, followed by rDIsSIVgag/pol induced an SIV-specific lymphoproliferative response that was mediated by a CD4+-T-lymphocyte subset. Immunization with either vaccine alone was insufficient to induce high levels of proliferation or Th1 responses in the animals. The prime-boost regimen also induced SIV Gag-specific cellular responses based on gamma interferon secretion, as well as cytotoxic-T-lymphocyte responses. Thus, the regimen of DNA priming and recombinant DIs boosting induced Th1-type cell-mediated immunity, which was associated with resistance to viral challenge with wild-type vaccinia virus expressing SIVgag/pol, suggesting that this new regimen may hold promise as a safe and effective vaccine against human immunodeficiency virus type 1.

* Corresponding author. Mailing address: AIDS Research Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: 81-3-5285-1111, ext. 2737. Fax: 81-3-5285-1183. E-mail: address: mhonda{at}nih.go.jp.

Journal of Virology, September 2004, p. 9842-9853, Vol. 78, No. 18
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.18.9842-9853.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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