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Journal of Virology, September 2004, p. 9807-9813, Vol. 78, No. 18
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.18.9807-9813.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Capsid Region Involved in Hepatitis A Virus Binding to Glycophorin A of the Erythrocyte Membrane

Glòria Sánchez,1 Lluís Aragonès,1 M. Isabel Costafreda,1 Enric Ribes,2 Albert Bosch,1* and Rosa M. Pintó1

Virus Entèrics, Department of Microbiology,1 Department of Cell Biology, University of Barcelona, Barcelona, Spain2

Received 22 January 2004/ Accepted 16 May 2004

Hepatitis A virus (HAV) has previously been reported to agglutinate human red blood cells at acidic pHs. Treatment of erythrocytes with different enzymes and chemical reagents indicated that HAV attachment is mediated through an interaction with sialylglycoproteins. HAV hemagglutination could be blocked by incubating the virus with glycophorin A, indicating that this sialylglycoprotein is the erythrocyte receptor. The number of receptors used was estimated to be around 500 per cell. At the same time, HAV-induced hemagglutination could also be blocked by either monoclonal antibody H7C27 or an anti-VP3(102-121) ascitic fluid, indicating that lysine 221 of VP1 and the surrounding VP3 residues lining the capsid pit are involved in HAV binding to erythrocytes.

* Corresponding author. Mailing address: Department of Microbiology, University of Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain. Phone: (34) 934034620. Fax: (34) 934034629. E-mail: abosch{at}ub.edu.

Journal of Virology, September 2004, p. 9807-9813, Vol. 78, No. 18
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.18.9807-9813.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Aragones, L., Bosch, A., Pinto, R. M. (2008). Hepatitis A Virus Mutant Spectra under the Selective Pressure of Monoclonal Antibodies: Codon Usage Constraints Limit Capsid Variability. J. Virol. 82: 1688-1700 [Abstract] [Full Text]  


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