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Retroviral Capsid Determinants of Fv1 NB and NR Tropism

Anthony Stevens,^1, Michael Bock,^1,, Scott Ellis,^1, Paul LeTissier,^1,¶ Kate N. Bishop,^1,|| Melvyn W. Yap,¹ Willie Taylor,² and Jonathan P. Stoye^1*

Divisions of Virology,¹ Mathematical Biology, National Institute for Medical Research, London, United Kingdom²

Received 17 February 2004/ Accepted 10 May 2004

The specificity determinants for susceptibility to resistance by the Fv1 n and b alleles map to amino acid 110 of the murine leukemia virus CA protein. To study the interaction between Fv1 and CA, we examined changes in CA resulting in the loss of susceptibility to Fv1 resistance in naturally occurring NB- and NR-tropic viruses. A variety of amino acid changes affecting Fv1 tropism were identified, at CA positions 82, 92 to 95, 105, 114, and 117, and they all were mapped to the apparent exterior of virion-associated CA. These amino acids may form a binding surface for Fv1.

A.S. and M. B. contributed equally to this study.

Present address: Institut fuer Virologie und Immunbiologie, Universitaet Wuerzburg, 97078 Wuerzburg, Germany.

Present address: BHF Molecular Cardiology Laboratory, Wellcome Trust Centre for Molecular Genetics, Headington, Oxford OX3 7BN, United Kingdom.

^¶ Present address: Division of Molecular Neuroendocrinology, National Institute for Medical Research, London NW7 1AA, United Kingdom.

^|| Present address: Department of Infectious Diseases, Guy's, King's and St. Thomas' Medical School, King's College London, London SE1 9RT, United Kingdom.

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