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Journal of Virology, September 2004, p. 9573-9578, Vol. 78, No. 17
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.17.9573-9578.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Mobility of the Human Immunodeficiency Virus (HIV) Receptor CD4 and Coreceptor CCR5 in Living Cells: Implications for HIV Fusion and Entry Events

Carolyn M. Steffens and Thomas J. Hope*

Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois

Received 7 February 2004/ Accepted 29 April 2004

The sequence of events leading to human immunodeficiency virus fusion and entry likely involves the recruitment of multiple receptor and coreceptor proteins to a specific complex by the viral envelope. Using fluorescence recovery after photobleaching technology, we find that both CD4 and CCR5 are mobile in the cell membrane. Interestingly, our findings also suggest that the seven-span transmembrane coreceptor is significantly more mobile than CD4 and requires membrane cholesterol for mobility.

* Corresponding author. Mailing address: Department of Microbiology and Immunology (MC 790), College of Medicine, University of Illinois at Chicago, E-704 Medical Sciences Building, 835 South Wolcott Ave., Chicago, IL 60612-7344. Phone: (312) 413-3424. Fax: (312) 996-6415. E-mail: thope{at}uic.edu.

Journal of Virology, September 2004, p. 9573-9578, Vol. 78, No. 17
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.17.9573-9578.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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