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Journal of Virology, September 2004, p. 9568-9572, Vol. 78, No. 17
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.17.9568-9572.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Model Suggesting that Replication of Influenza Virus Is Regulated by Stabilization of Replicative Intermediates{dagger}

Frank T. Vreede, Tanis E. Jung, and George G. Brownlee*

Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

Received 10 March 2004/ Accepted 26 April 2004

The RNA-dependent RNA polymerase of influenza A virus is responsible for both transcription and replication of negative-sense viral RNA. It is thought that a "switching" mechanism regulates the transition between these activities. We demonstrate that, in the presence of preexisting viral RNA polymerase and nucleoprotein (NP), influenza A virus synthesizes both mRNA (transcription) and cRNA (replication) early in infection. We suggest that there may be no switch regulating the initiation of RNA synthesis and present a model suggesting that nascent cRNA is degraded by host cell nucleases unless it is stabilized by newly synthesized viral RNA polymerase and NP.

* Corresponding author. Mailing address: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom. Phone: 44-1865-275559. Fax: 44-1865-275556. E-mail: george.brownlee{at}path.ox.ac.uk.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, September 2004, p. 9568-9572, Vol. 78, No. 17
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.17.9568-9572.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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