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Journal of Virology, August 2004, p. 8935-8941, Vol. 78, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.16.8935-8941.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Gene Encoding the Nucleocapsid Protein of Gill-Associated Nidovirus of Penaeus monodon Prawns Is Located Upstream of the Glycoprotein Gene

Jeff A. Cowley,1* Lee C. Cadogan,1 Kirsten M. Spann,1,{dagger} Nusra Sittidilokratna,2 and Peter J. Walker1

CSIRO Livestock Industries, Queensland Bioscience Precinct, St. Lucia 4067, Australia,1 CENTEX Shrimp, Faculty of Science, Mahidol University, Bangkok 10400, Thailand2

Received 18 August 2003/ Accepted 6 April 2004

The ORF2 gene of Gill-associated virus (GAV) of Penaeus monodon prawns resides 93 nucleotides downstream of the ORF1a-ORF1b gene and encodes a 144-amino-acid hydrophilic polypeptide (15,998 Da; pI, 9.75) containing 20 basic (14%) and 13 acidic (9%) residues and 19 prolines (13%). Antiserum to a synthetic ORF2 peptide or an Escherichia coli-expressed glutathione S-transferase-ORF2 fusion protein detected a 20-kDa protein in infected lymphoid organ and gill tissues in Western blots. The GAV ORF2 fusion protein antiserum also cross-reacted with the p20 nucleoprotein in virions of the closely related Yellow head virus. By immuno-gold electron microscopy, it was observed that the ORF2 peptide antibody localized to tubular GAV nucleocapsids, often at the ends or at lateral cross sections. As GAV appears to contain only two structural protein genes (ORF2 and ORF3), these data indicate that GAV differs from vertebrate nidoviruses in that the gene encoding the nucleocapsid protein is located upstream of the gene encoding the virion glycoproteins.

* Corresponding author. Mailing address: CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Rd., St. Lucia 4067, Australia. Phone: 61-7-3214-2527. Fax: 61-7-3214-2900. E-mail: Jeff.Cowley{at}csiro.au.

{dagger} Present address: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892-0720.

Journal of Virology, August 2004, p. 8935-8941, Vol. 78, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.16.8935-8941.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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