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Journal of Virology, August 2004, p. 8931-8934, Vol. 78, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.16.8931-8934.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

RNA Interference Directed against Poly(ADP-Ribose) Polymerase 1 Efficiently Suppresses Human Immunodeficiency Virus Type 1 Replication in Human Cells

Masanori Kameoka,1* Souichi Nukuzuma,2 Asako Itaya,1 Yasuharu Tanaka,1 Katsuya Ota,1 Kazuyoshi Ikuta,3 and Koichiro Yoshihara1

Department of Biochemistry, Nara Medical University, Kashihara, Nara 634-8521,1 Department of Microbiology, Kobe Institute of Health, Kobe, Hyogo 650-0046,2 Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan3

Received 4 February 2004/ Accepted 12 April 2004

We established small interfering RNA (siRNA) directed against poly(ADP-ribose) polymerase 1 (PARP-1) that effectively reduces the expression of PARP-1 in two human cell lines. Established siRNA against PARP-1 significantly suppressed human immunodeficiency virus type 1 (HIV-1) replication, as well as the activation of the integrated HIV-1 long terminal repeat promoter. These results indicate that PARP-1 is required for efficient HIV-1 replication in human cells. We propose that PARP-1 may serve as a cellular target for RNA interference-mediated gene silencing to inhibit HIV-1 replication.

* Corresponding author. Mailing address: Department of Biochemistry, Nara Medical University, Shijo 840, Kashihara, Nara 364-8521, Japan. Phone: 81 (744) 29-8837. Fax: 81 (744) 29-7176. E-mail: mkameoka{at}naramed-u.ac.jp.

Journal of Virology, August 2004, p. 8931-8934, Vol. 78, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.16.8931-8934.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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