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Journal of Virology, August 2004, p. 8382-8391, Vol. 78, No. 15
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.15.8382-8391.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Human Cytomegalovirus-Encoded G Protein-Coupled Receptor US28 Mediates Smooth Muscle Cell Migration through G{alpha}12

Ryan M. Melnychuk,{dagger} Daniel N. Streblow,{dagger}* Patricia P. Smith, Alec J. Hirsch, Dora Pancheva, and Jay A. Nelson

Department of Molecular Microbiology and Immunology and Vaccine and Gene Therapy Institute, Oregon Health Sciences University, Portland, Oregon 97201

Received 10 November 2003/ Accepted 3 March 2004

Coupling of G proteins to ligand-engaged chemokine receptors is the paramount event in G-protein-coupled receptor signal transduction. Previously, we have demonstrated that the human cytomegalovirus-encoded chemokine receptor US28 mediates human vascular smooth muscle cell (SMC) migration in response to either RANTES or monocyte chemoattractant protein 1. In this report, we identify the G proteins that couple with US28 to promote vascular SMC migration and identify other signaling molecules that play critical roles in this process. US28-mediated cellular migration was enhanced with the expression of the G-protein subunits G{alpha}12 and G{alpha}13, suggesting that US28 may functionally couple to these G proteins. In correlation with this observation, US28 was able to activate RhoA, a downstream effector of G{alpha}12 and G{alpha}13 in cell types with these G proteins but not in those without them and activation of RhoA was dependent on US28 stimulation with RANTES. In addition, inactivation of RhoA or the RhoA-associated kinase p160ROCK with a dominant-negative mutant of RhoA or the small molecule inhibitor Y27632, respectively, abrogated US28-induced SMC migration. The data presented here suggest that US28 functionally signals through G{alpha}12 family G proteins and RhoA in a ligand-dependent manner and these signaling molecules are important for the ability of US28 to induce cellular migration.

* Corresponding author. Mailing address: Molecular Microbiology and Immunology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd., Portland, OR 97201. Phone: (503) 494-2440. Fax: (503) 494-6862. E-mail: streblow{at}ohsu.edu.

{dagger} R.M.M. and D.N.S. contributed equally to the manuscript.

Journal of Virology, August 2004, p. 8382-8391, Vol. 78, No. 15
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.15.8382-8391.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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