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Journal of Virology, August 2004, p. 8349-8358, Vol. 78, No. 15
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.15.8349-8358.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Global Identification of Three Major Genotypes of Varicella-Zoster Virus: Longitudinal Clustering and Strategies for Genotyping

Vladimir N. Loparev,1* Antonio Gonzalez,1 Marlene Deleon-Carnes,1 Graham Tipples,2 Helmut Fickenscher,3 Einar G. Torfason,4 and D. Scott Schmid1*

National VZV Laboratory, Herpesvirus Section, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,1 National Microbiology Laboratory, Winnipeg, Canada,2 Virology Department, Ruprecht-Karls University, Heidelberg, Germany,3 Department of Medical Virology, Landspitali University Hospital, Reyjavik, Iceland4

Received 16 October 2003/ Accepted 24 March 2004

By analysis of a single, variable, and short DNA sequence of 447 bp located within open reading frame 22 (ORF22), we discriminated three major varicella-zoster virus (VZV) genotypes. VZV isolates from all six inhabited continents that showed nearly complete homology to ORF22 of the European reference strain Dumas were assigned to the European (E) genotype. All Japanese isolates, defined as the Japanese (J) genotype, were identical in the respective genomic region and proved the most divergent from the E strains, carrying four distinct variations. The remaining isolates carried a combination of E- and J-specific variations in the target sequence and thus were collectively termed the mosaic (M) genotype. Three hundred twenty-six isolates collected in 27 countries were genotyped. A distinctive longitudinal distribution of VZV genotypes supports this approach. Among 111 isolates collected from European patients, 96.4% were genotype E. Consistent with this observation, approximately 80% of the VZV strains from the United States were also genotype E. Similarly, genotype E viruses were dominant in the Asian part of Russia and in eastern Australia. M genotype viruses were strongly dominant in tropical regions of Africa, Indochina, and Central America, and they were common in western Australia. However, genotype M viruses were also identified as a minority in several countries worldwide. Two major intertypic variations of genotype M strains were identified, suggesting that the M genotype can be further differentiated into subgenotypes. These data highlight the direction for future VZV genotyping efforts. This approach provides the first simple genotyping method for VZV strains in clinical samples.

* Corresponding author. Mailing address: CDC/NCID/DVRD, 1600 Clifton Rd., MS G-18, Atlanta, GA 30333. Phone for Vladimir N. Loparev: (404) 639-4040. Fax: (404) 639-4056. E-mail: vnl0{at}cdc.gov. Phone for D. Scott Schmidt: (404) 639-0066. Fax: (404) 639-4056. E-mail: dss1{at}cdc.gov.

Journal of Virology, August 2004, p. 8349-8358, Vol. 78, No. 15
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.15.8349-8358.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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