This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Irie, T.
Right arrow Articles by Harty, R. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Irie, T.
Right arrow Articles by Harty, R. N.

 Previous Article  |  Next Article 

Journal of Virology, July 2004, p. 7823-7827, Vol. 78, No. 14
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.14.7823-7827.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Functional Analysis of Late-Budding Domain Activity Associated with the PSAP Motif within the Vesicular Stomatitis Virus M Protein

Takashi Irie,1 Jillian M. Licata,1 Himangi R. Jayakar,2 Michael A. Whitt,2,3 Peter Bell,4 and Ronald N. Harty1*

Department of Pathobiology, School of Veterinary Medicine,1 Cell Morphology Core, Gene Therapy Program, Division of Medical Genetics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104,4 GTx, Inc.,2 Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 381633

Received 21 January 2004/ Accepted 24 February 2004

A PPPY motif within the M protein of vesicular stomatitis virus (VSV) functions as a late-budding domain (L-domain); however, L-domain activity has yet to be associated with a downstream PSAP motif. VSV recombinants with mutations in the PPPY and/or PSAP motif were recovered by reverse genetics and examined for growth kinetics, plaque size, and budding efficiency by electron microscopy. Results indicate that unlike the PPPY motif, the PSAP motif alone does not possess L-domain activity. Finally, the insertion of the human immunodeficiency virus type 1 p6 L-domain and flanking sequences into the PSAP region of M protein rescued budding of a PPPY mutant of VSV to wild-type levels.

* Corresponding author. Mailing address: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St., Philadelphia, PA 19104-6049. Phone: (215) 573-4485. Fax: (215) 898-7887. E-mail: rharty{at}vet.upenn.edu.

Journal of Virology, July 2004, p. 7823-7827, Vol. 78, No. 14
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.14.7823-7827.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dancho, B., McKenzie, M. O., Connor, J. H., Lyles, D. S. (2009). Vesicular Stomatitis Virus Matrix Protein Mutations That Affect Association with Host Membranes and Viral Nucleocapsids. J. Biol. Chem. 284: 4500-4509 [Abstract] [Full Text]  
  • Gholami, A., Kassis, R., Real, E., Delmas, O., Guadagnini, S., Larrous, F., Obach, D., Prevost, M.-C., Jacob, Y., Bourhy, H. (2008). Mitochondrial Dysfunction in Lyssavirus-Induced Apoptosis. J. Virol. 82: 4774-4784 [Abstract] [Full Text]  
  • Taylor, G. M., Hanson, P. I., Kielian, M. (2007). Ubiquitin Depletion and Dominant-Negative VPS4 Inhibit Rhabdovirus Budding without Affecting Alphavirus Budding. J. Virol. 81: 13631-13639 [Abstract] [Full Text]  
  • Irie, T., Carnero, E., Okumura, A., Garcia-Sastre, A., Harty, R. N. (2007). Modifications of the PSAP region of the matrix protein lead to attenuation of vesicular stomatitis virus in vitro and in vivo. J. Gen. Virol. 88: 2559-2567 [Abstract] [Full Text]  
  • Marston, D. A., McElhinney, L. M., Johnson, N., Muller, T., Conzelmann, K. K., Tordo, N., Fooks, A. R. (2007). Comparative analysis of the full genome sequence of European bat lyssavirus type 1 and type 2 with other lyssaviruses and evidence for a conserved transcription termination and polyadenylation motif in the G-L 3' non-translated region. J. Gen. Virol. 88: 1302-1314 [Abstract] [Full Text]  
  • Irie, T., Shimazu, Y., Yoshida, T., Sakaguchi, T. (2007). The YLDL Sequence within Sendai Virus M Protein Is Critical for Budding of Virus-Like Particles and Interacts with Alix/AIP1 Independently of C Protein. J. Virol. 81: 2263-2273 [Abstract] [Full Text]  
  • Irie, T., Harty, R. N. (2005). L-Domain Flanking Sequences Are Important for Host Interactions and Efficient Budding of Vesicular Stomatitis Virus Recombinants. J. Virol. 79: 12617-12622 [Abstract] [Full Text]  
  • Neumann, G., Ebihara, H., Takada, A., Noda, T., Kobasa, D., Jasenosky, L. D., Watanabe, S., Kim, J. H., Feldmann, H., Kawaoka, Y. (2005). Ebola Virus VP40 Late Domains Are Not Essential for Viral Replication in Cell Culture. J. Virol. 79: 10300-10307 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»