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Journal of Virology, July 2004, p. 7818-7822, Vol. 78, No. 14
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.14.7818-7822.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Bromodeoxyuridine-Labeled Viral Particles as a Tool for Visualization of the Immediate-Early Events of Human Cytomegalovirus Infection

Department of Microbiology, Molecular Biology and Biochemistry and The Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3052

Received 1 December 2003/ Accepted 17 March 2004

We describe here a simple method for labeling the genome of human cytomegalovirus, a large double-stranded DNA virus, with bromodeoxyuridine (BrdU). The labeled DNA was incorporated into viral particles, which were then collected in cell supernatant. To demonstrate the versatility and effectiveness of this method, labeled virions were used to study the immediate-early events of virus-host cell interaction via indirect immunofluorescence microscopy. It is our hope that this new methodology will prove useful in the study of binding, entry and viral genome deposition in diverse virus systems.

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