This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rosenke, K.
Right arrow Articles by Fortunato, E. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rosenke, K.
Right arrow Articles by Fortunato, E. A.

 Previous Article  |  Next Article 

Journal of Virology, July 2004, p. 7818-7822, Vol. 78, No. 14
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.14.7818-7822.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Bromodeoxyuridine-Labeled Viral Particles as a Tool for Visualization of the Immediate-Early Events of Human Cytomegalovirus Infection

Kyle Rosenke and Elizabeth A. Fortunato*

Department of Microbiology, Molecular Biology and Biochemistry and The Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3052

Received 1 December 2003/ Accepted 17 March 2004

We describe here a simple method for labeling the genome of human cytomegalovirus, a large double-stranded DNA virus, with bromodeoxyuridine (BrdU). The labeled DNA was incorporated into viral particles, which were then collected in cell supernatant. To demonstrate the versatility and effectiveness of this method, labeled virions were used to study the immediate-early events of virus-host cell interaction via indirect immunofluorescence microscopy. It is our hope that this new methodology will prove useful in the study of binding, entry and viral genome deposition in diverse virus systems.

* Corresponding author. Mailing address: University of Idaho, Dept. of Microbiology, Molecular Biology and Biochemistry, LSS 142, Moscow, ID 83844-3052. Phone: (208) 885-6966. Fax: (208) 885-6518. E-mail: lfort{at}uidaho.edu.

Journal of Virology, July 2004, p. 7818-7822, Vol. 78, No. 14
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.14.7818-7822.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lukashchuk, V., McFarlane, S., Everett, R. D., Preston, C. M. (2008). Human Cytomegalovirus Protein pp71 Displaces the Chromatin-Associated Factor ATRX from Nuclear Domain 10 at Early Stages of Infection. J. Virol. 82: 12543-12554 [Abstract] [Full Text]  
  • Luo, M. H., Fortunato, E. A. (2007). Long-Term Infection and Shedding of Human Cytomegalovirus in T98G Glioblastoma Cells. J. Virol. 81: 10424-10436 [Abstract] [Full Text]  
  • Luo, M. H., Rosenke, K., Czornak, K., Fortunato, E. A. (2007). Human Cytomegalovirus Disrupts both Ataxia Telangiectasia Mutated Protein (ATM)- and ATM-Rad3-Related Kinase-Mediated DNA Damage Responses during Lytic Infection. J. Virol. 81: 1934-1950 [Abstract] [Full Text]  
  • Cantrell, S. R., Bresnahan, W. A. (2006). Human Cytomegalovirus (HCMV) UL82 Gene Product (pp71) Relieves hDaxx-Mediated Repression of HCMV Replication.. J. Virol. 80: 6188-6191 [Abstract] [Full Text]  
  • Preston, C. M., Nicholl, M. J. (2006). Role of the cellular protein hDaxx in human cytomegalovirus immediate-early gene expression.. J. Gen. Virol. 87: 1113-1121 [Abstract] [Full Text]  
  • Tamrakar, S., Kapasi, A. J., Spector, D. H. (2005). Human Cytomegalovirus Infection Induces Specific Hyperphosphorylation of the Carboxyl-Terminal Domain of the Large Subunit of RNA Polymerase II That Is Associated with Changes in the Abundance, Activity, and Localization of cdk9 and cdk7. J. Virol. 79: 15477-15493 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»