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Journal of Virology, July 2004, p. 7284-7287, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.7284-7287.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Ex Vivo Phenotype and Frequency of Influenza Virus-Specific CD4 Memory T Cells

Michaela Lucas,1,{dagger} Cheryl L. Day,1,{dagger} Jessica R. Wyer,2 Sharon L. Cunliffe,2 Andrew Loughry,1 Andrew J. McMichael,2 and Paul Klenerman1*

Peter Medawar Building, Nuffield Department of Medicine, University of Oxford,1 MRC Human Immunology Unit, W.I.M.M., John Radcliffe Hospital, Oxford, United Kingdom2

Received 23 December 2003/ Accepted 19 February 2004

Recent advances in class II tetramer staining technology have allowed reliable direct ex vivo visualization of antigen-specific CD4 T cells. In order to define the frequency and phenotype of a prototype response to a nonpersistent pathogen, we have used such techniques to analyze influenza virus-specific memory CD4 T cells directly from blood. These responses are stably detectable ex vivo at low frequencies (range, 0.00012 to 0.0061% of CD4 T cells) and display a distinct "central memory" CD62L+ phenotype.

* Corresponding author. Mailing address: Peter Medawar Building, Nuffield Department of Medicine, University of Oxford, South Parks Road, Oxford OX1 3SY, United Kingdom. Phone: 44 1865 281885. Fax: 44 1865 281236. E-mail: paul.klenerman{at}medawar.oxford.ac.uk.

{dagger} M.L. and C.L.D. contributed equally to this work.

Journal of Virology, July 2004, p. 7284-7287, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.7284-7287.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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