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Journal of Virology, July 2004, p. 7270-7273, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.7270-7273.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Inhibition of Virus Production in JC Virus-Infected Cells by Postinfection RNA Interference

Yasuko Orba,1,2 Hirofumi Sawa,1,2,3* Hiroshi Iwata,1,2 Shinya Tanaka,1,2 and Kazuo Nagashima1,2

Laboratory of Molecular and Cellular Pathology,1 21st Century COE Program for Zoonosis Control, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo 060-8638 ,3 CREST, JST, Sapporo, Japan2

Received 25 February 2004/ Accepted 30 March 2004

RNA interference has been applied for the prevention of virus infections in mammalian cells but has not succeeded in eliminating infections from already infected cells. We now show that the transfection of JC virus-infected SVG-A human glial cells with small interfering RNAs that target late viral proteins, including agnoprotein and VP1, results in a marked inhibition both of viral protein expression and of virus production. RNA interference directed against JC virus genes may thus provide a basis for the development of new strategies to control infections with this polyomavirus.

* Corresponding author. Mailing address: Laboratory of Molecular and Cellular Pathology, Hokkaido University School of Medicine, N15, W7, Kita-ku, Sapporo 060-8638, Japan. Phone: 81-11-706-5053. Fax: 81-11-706-7806. E-mail: h-sawa{at}patho2.med.hokudai.ac.jp.

Journal of Virology, July 2004, p. 7270-7273, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.7270-7273.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • White, M. K., Safak, M., Khalili, K. (2009). Regulation of Gene Expression in Primate Polyomaviruses. J. Virol. 83: 10846-10856 [Abstract] [Full Text]  
  • Suzuki, T., Okada, Y., Semba, S., Orba, Y., Yamanouchi, S., Endo, S., Tanaka, S., Fujita, T., Kuroda, S., Nagashima, K., Sawa, H. (2005). Identification of FEZ1 as a Protein That Interacts with JC Virus Agnoprotein and Microtubules: ROLE OF AGNOPROTEIN-INDUCED DISSOCIATION OF FEZ1 FROM MICROTUBULES IN VIRAL PROPAGATION. J. Biol. Chem. 280: 24948-24956 [Abstract] [Full Text]  
  • Shankar, P., Manjunath, N., Lieberman, J. (2005). The Prospect of Silencing Disease Using RNA Interference. JAMA 293: 1367-1373 [Abstract] [Full Text]  


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