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Journal of Virology, July 2004, p. 7148-7152, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.7148-7152.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Prion Infection of Epithelial Rov Cells Is a Polarized Event

Sophie Paquet,1 Elifsu Sabuncu,1 Jean-Louis Delaunay,2 Hubert Laude,1 and Didier Vilette1*

Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas,1 INSERMU538, Faculté de Médecine Saint-Antoine, 75571 Paris Cedex 12, France2

Received 23 December 2003/ Accepted 19 February 2004

During prion infections, the cellular glycosylphosphatidylinositol-anchored glycoprotein PrP is converted into a conformational isoform. This abnormal conformer is thought to recruit and convert the normal cellular PrP into a likeness of itself and is proposed to be the infectious agent. We investigated the distribution of the PrP protein on the surface of Rov cells, an epithelial cell line highly permissive to prion multiplication, and we found that PrP is primarily expressed on the apical side. We further show that prion transmission to Rov cells is much more efficient if infectivity contacts the apical side, indicating that the apical and basolateral sides of Rov cells are not equally competent for prion infection and adding prions to the list of the conventional infectious agents (viruses and bacteria) that infect epithelial cells in a polarized manner. These data raise the possibility that apically expressed PrP may be involved in this polarized process of infection. This would add further support for a crucial role of PrP at the cell surface in prion infection of target cells.

* Corresponding author. Mailing address: Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France. Phone: 33134652616. Fax: 33134652621. E-mail: vilette{at}jouy.inra.fr.

Journal of Virology, July 2004, p. 7148-7152, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.7148-7152.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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