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Journal of Virology, July 2004, p. 6875-6882, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.6875-6882.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of Infectious Retroviral Pseudotype Particles Bearing Hepatitis C Virus Glycoproteins

Mike Flint, Carine Logvinoff, Charles M. Rice, and Jane A. McKeating*

Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10021

Received 1 January 2004/ Accepted 28 February 2004

The recent development of infectious retroviral pseudotypes bearing hepatitis C virus (HCV) glycoproteins represents an opportunity to study the functionally active form of the HCV E1 and E2 glycoproteins. In the culture supernatant of cells producing HCV retroviral pseudotypes, the majority of E2 was not associated with infectious particles and failed to sediment on sucrose gradients. The E2 that was incorporated into infectious particles appeared as a triplet of diffuse bands at 60, 70, and 90 kDa. Similarly, three major forms of E1 were incorporated into the pseudotype particles, migrating at 33, 31, and 25 kDa. Endoglycosidase H (endo-H) treatment of particles demonstrated that the incorporated E1 was partially or completely sensitive to digestion. In contrast, the majority of the incorporated E2 was endo-H resistant. Purified pseudotype particles were found to contain both disulfide-linked aggregates and nonaggregated E1 and E2. HCV pseudotypes generated from cells expressing E1E2p7 showed similar heterogeneity in the incorporated glycoproteins and were of comparable infectivity to those generated by expression of E1E2. Our results demonstrate the heterogenous nature of E1 and E2 incorporated into retroviral pseudotypes and highlight the difficulty in identifying forms of the HCV glycoproteins that initiate infection.

* Corresponding author. Mailing address: The Center for the Study of Hepatitis C, The Rockefeller University, Box 64, 1230 York Ave., New York, NY 10021. Phone: (212) 327-7066. Fax: (212) 327-7047. E-mail: mckeatj{at}mail.rockefeller.edu.

Journal of Virology, July 2004, p. 6875-6882, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.6875-6882.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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