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Cleavage Inhibition of the Murine Coronavirus Spike Protein by a Furin-Like Enzyme Affects Cell-Cell but Not Virus-Cell Fusion

Cornelis A. M. de Haan, Konrad Stadler,^, Gert-Jan Godeke, Berend Jan Bosch, and Peter J. M. Rottier^*

Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine and Institute of Biomembranes, Utrecht University, 3584 CL Utrecht, The Netherlands

Received 30 September 2003/ Accepted 3 February 2004

Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.

K.S. and C.A.M.D.H. contributed equally to this work.

Present address: IRIS, Chiron S.r.l., 53100 Siena, Italy.

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