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Journal of Virology, May 2003, p. 5519-5523, Vol. 77, No. 9
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.9.5519-5523.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role of N Glycosylation of Hepatitis B Virus Envelope Proteins in Morphogenesis and Infectivity of Hepatitis Delta Virus

Camille Sureau,1,2* Chantal Fournier-Wirth,3 and Patrick Maurel4

Laboratoire de Virologie Moléculaire, INSERM U76, INTS, Paris,1 Etablissement Français du Sang,3 INSERM U128, Montpellier, France,4 Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 782282

Received 25 November 2002/ Accepted 24 January 2003

Hepatitis delta virus (HDV) particles are coated with the large (L), middle (M), and small (S) hepatitis B virus envelope proteins. In the present study, we constructed glycosylation-defective envelope protein mutants and evaluated their capacity to assist in the maturation of infectious HDV in vitro. We observed that the removal of N-linked carbohydrates on the S, M, and L proteins was tolerated for the assembly of subviral hepatitis B virus (HBV) particles but was partially inhibitory for the formation of HDV virions. However, when assayed on primary cultures of human hepatocytes, virions coated with S, M, and L proteins lacking N-linked glycans were infectious. Furthermore, in the absence of M, HDV particles coated with nonglycosylated S and L proteins retained infectivity. These results indicate that carbohydrates on the HBV envelope proteins are not essential for the in vitro infectivity of HDV.

* Corresponding author. Mailing address: Laboratoire de Virologie Moléculaire, INSERM U76, Institut National de la Transfusion Sanguine, 6 Rue Alexandre-Cabanel, 75739 Paris, France. Phone: (33) 1 44 49 30 56. Fax: (33) 1 43 06 50 19. E-mail: csureau{at}ints.fr.

Journal of Virology, May 2003, p. 5519-5523, Vol. 77, No. 9
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.9.5519-5523.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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