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Journal of Virology, May 2003, p. 5084-5097, Vol. 77, No. 9
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.9.5084-5097.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Analysis of 4.3 Kilobases of Divergent Locus B of Macaque Retroperitoneal Fibromatosis-Associated Herpesvirus Reveals a Close Similarity in Gene Sequence and Genome Organization to Kaposi's Sarcoma-Associated Herpesvirus

Timothy M. Rose,1,2* Jonathan T. Ryan,1 Emily R. Schultz,1,{dagger} Brian W. Raden,1,{ddagger} and Che-Chung Tsai2

Department of Pathobiology, School of Public Health and Community Medicine,1 National Primate Research Center, University of Washington, Seattle, Washington2

Received 2 December 2002/ Accepted 28 January 2003

We previously identified retroperitoneal fibromatosis-associated herpesvirus (RFHV) as a simian homolog of Kaposi's sarcoma-associated herpesvirus (KSHV) in a fibroproliferative malignancy of macaques that has similarities to Kaposi's sarcoma. In this report, we cloned 4.3 kb of divergent locus B (DL-B) flanking the DNA polymerase gene from two variants of RFHV from different species of macaque with a consensus degenerate hybrid oligonucleotide primer approach. Within the DL-B region of RFHV, viral homologs of the cellular interleukin-6, dihydrofolate reductase, and thymidylate synthase genes were identified, along with a homolog of the gammaherpesvirus open reading frame (ORF) 10. In addition, a homolog of the KSHV ORF K3, the modulator of immune recognition-1, was identified. Our data show a close similarity in sequence conservation, gene content, and genomic structure between RFHV and KSHV which strongly supports the grouping of these viral species within the same RV-1 rhadinovirus lineage and the hypothesis that RFHV is the macaque homolog of KSHV.

* Corresponding author. Mailing address: Department of Pathobiology, Box 357238, University of Washington, Seattle, WA 98195. Phone: (206) 616-2084. Fax: (206) 543-3873. E-mail: trose{at}u.washington.edu.

{dagger} Present address: Rosetta Inpharmatics, Inc., Bothell, WA 98011.

{ddagger} Present address: Fred Hutchinson Cancer Research Center, Seattle, WA 98109.

Journal of Virology, May 2003, p. 5084-5097, Vol. 77, No. 9
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.9.5084-5097.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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