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Journal of Virology, April 2003, p. 5017-5020, Vol. 77, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.8.5017-5020.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Single Amino Acid Mutation in the PA Subunit of the Influenza Virus RNA Polymerase Promotes the Generation of Defective Interfering RNAs

Ervin Fodor, Louise J. Mingay, Mandy Crow, Tao Deng, and George G. Brownlee*

Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

Received 18 October 2002/ Accepted 24 January 2003

An R638A mutation of the polymerase acidic protein (PA) subunit of the RNA polymerase of influenza A/WSN/33 virus results in severe attenuation of viral growth in cell culture by promoting the synthesis of defective interfering RNAs. We propose that R638A is an "elongation" mutant that destabilizes PA-RNA template interactions during elongation. A C453R mutation in PA can compensate for this defect, suggesting that amino acids C453 and R638 form part of the same domain.

* Corresponding author. Mailing address: Sir William Dunn School of Pathology, University of Oxford, South Parks Rd., Oxford OX1 3RE, United Kingdom. Phone: 44 (01865) 275559. Fax: 44 (01865) 275556. E-mail: George.Brownlee{at}path.ox.ac.uk.

Journal of Virology, April 2003, p. 5017-5020, Vol. 77, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.8.5017-5020.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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