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Journal of Virology, April 2003, p. 4985-4991, Vol. 77, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.8.4985-4991.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Localization of CD4 and CCR5 in Living Cells

Carolyn M. Steffens and Thomas J. Hope*

Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois

Received 18 October 2002/ Accepted 13 January 2003

The events preceding human immunodeficiency virus fusion and entry are influenced by the concentration and distribution of receptor and coreceptor molecules on the cell surface. However, the extent to which these proteins colocalize with one another in the cell membrane remains unclear. Using high-resolution deconvolution fluorescent microscopy of living cells, we found that both CD4 and CCR5 accumulate in protruding membrane structures containing actin and ezrin. Although CD4 and CCR5 extensively colocalize in these structures, they do not exist in a stable complex.

* Corresponding author. Mailing address: Department of Microbiology and Immunology (MC 790), College of Medicine, University of Illinois at Chicago, E-704 Medical Sciences Building, 835 South Wolcott Ave., Chicago, IL 60612. Phone: (312) 413-3424. Fax: (312) 996-6415. E-mail: thope{at}uic.edu.

Journal of Virology, April 2003, p. 4985-4991, Vol. 77, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.8.4985-4991.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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