This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Navas, S.
Right arrow Articles by Weiss, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Navas, S.
Right arrow Articles by Weiss, S. R.

 Previous Article  |  Next Article 

Journal of Virology, April 2003, p. 4972-4978, Vol. 77, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.8.4972-4978.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Murine Coronavirus-Induced Hepatitis: JHM Genetic Background Eliminates A59 Spike-Determined Hepatotropism

Sonia Navas and Susan R. Weiss*

Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6076

Received 10 December 2002/ Accepted 28 January 2003

Recombinant murine coronaviruses, differing only in the spike gene and containing the strain A59 (moderately hepatotropic) and JHM (neurotropic) spike genes in the background of the JHM genome, were compared for the ability to replicate in the liver and induce hepatitis in weanling C57BL/6 mice. Interestingly, expression of the A59 spike glycoprotein within the background of the neurotropic JHM strain does not reproduce the A59 hepatotropic phenotype. Thus, the JHM genetic background plays a dominant role over the spike in the determination of hepatotropism.

* Corresponding author. Mailing address: Department of Microbiology, University of Pennsylvania School of Medicine, 36th St. and Hamilton Walk, Philadelphia, PA 19104-6076. Phone: (215) 898-8013. Fax: (215) 573-4858. E-mail: weisssr{at}mail.med.upenn.edu.

Journal of Virology, April 2003, p. 4972-4978, Vol. 77, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.8.4972-4978.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Roth-Cross, J. K., Stokes, H., Chang, G., Chua, M. M., Thiel, V., Weiss, S. R., Gorbalenya, A. E., Siddell, S. G. (2009). Organ-Specific Attenuation of Murine Hepatitis Virus Strain A59 by Replacement of Catalytic Residues in the Putative Viral Cyclic Phosphodiesterase ns2. J. Virol. 83: 3743-3753 [Abstract] [Full Text]  
  • Roth-Cross, J. K., Bender, S. J., Weiss, S. R. (2008). Murine Coronavirus Mouse Hepatitis Virus Is Recognized by MDA5 and Induces Type I Interferon in Brain Macrophages/Microglia. J. Virol. 82: 9829-9838 [Abstract] [Full Text]  
  • MacNamara, K. C., Bender, S. J., Chua, M. M., Watson, R., Weiss, S. R. (2008). Priming of CD8+ T Cells during Central Nervous System Infection with a Murine Coronavirus Is Strain Dependent. J. Virol. 82: 6150-6160 [Abstract] [Full Text]  
  • Miura, T. A., Travanty, E. A., Oko, L., Bielefeldt-Ohmann, H., Weiss, S. R., Beauchemin, N., Holmes, K. V. (2008). The Spike Glycoprotein of Murine Coronavirus MHV-JHM Mediates Receptor-Independent Infection and Spread in the Central Nervous Systems of Ceacam1a / Mice. J. Virol. 82: 755-763 [Abstract] [Full Text]  
  • Roth-Cross, J. K., Martinez-Sobrido, L., Scott, E. P., Garcia-Sastre, A., Weiss, S. R. (2007). Inhibition of the Alpha/Beta Interferon Response by Mouse Hepatitis Virus at Multiple Levels. J. Virol. 81: 7189-7199 [Abstract] [Full Text]  
  • Goebel, S. J., Miller, T. B., Bennett, C. J., Bernard, K. A., Masters, P. S. (2007). A Hypervariable Region within the 3' cis-Acting Element of the Murine Coronavirus Genome Is Nonessential for RNA Synthesis but Affects Pathogenesis. J. Virol. 81: 1274-1287 [Abstract] [Full Text]  
  • Navas-Martin, S., Brom, M., Chua, M.-M., Watson, R., Qiu, Z., Weiss, S. R. (2007). Replicase Genes of Murine Coronavirus Strains A59 and JHM Are Interchangeable: Differences in Pathogenesis Map to the 3' One-Third of the Genome. J. Virol. 81: 1022-1026 [Abstract] [Full Text]  
  • De Albuquerque, N., Baig, E., Ma, X., Zhang, J., He, W., Rowe, A., Habal, M., Liu, M., Shalev, I., Downey, G. P., Gorczynski, R., Butany, J., Leibowitz, J., Weiss, S. R., McGilvray, I. D., Phillips, M. J., Fish, E. N., Levy, G. A. (2006). Murine hepatitis virus strain 1 produces a clinically relevant model of severe acute respiratory syndrome in a/j mice.. J. Virol. 80: 10382-10394 [Abstract] [Full Text]  
  • Iacono, K. T., Kazi, L., Weiss, S. R. (2006). Both Spike and Background Genes Contribute to Murine Coronavirus Neurovirulence. J. Virol. 80: 6834-6843 [Abstract] [Full Text]  
  • Kazi, L., Lissenberg, A., Watson, R., de Groot, R. J., Weiss, S. R. (2005). Expression of Hemagglutinin Esterase Protein from Recombinant Mouse Hepatitis Virus Enhances Neurovirulence. J. Virol. 79: 15064-15073 [Abstract] [Full Text]  
  • Weiss, S. R., Navas-Martin, S. (2005). Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus. Microbiol. Mol. Biol. Rev. 69: 635-664 [Abstract] [Full Text]  
  • Peti, W., Johnson, M. A., Herrmann, T., Neuman, B. W., Buchmeier, M. J., Nelson, M., Joseph, J., Page, R., Stevens, R. C., Kuhn, P., Wuthrich, K. (2005). Structural Genomics of the Severe Acute Respiratory Syndrome Coronavirus: Nuclear Magnetic Resonance Structure of the Protein nsP7. J. Virol. 79: 12905-12913 [Abstract] [Full Text]  
  • MacNamara, K. C., Chua, M. M., Phillips, J. J., Weiss, S. R. (2005). Contributions of the Viral Genetic Background and a Single Amino Acid Substitution in an Immunodominant CD8+ T-Cell Epitope to Murine Coronavirus Neurovirulence. J. Virol. 79: 9108-9118 [Abstract] [Full Text]  
  • Navas-Martin, S., Hingley, S. T., Weiss, S. R. (2005). Murine Coronavirus Evolution In Vivo: Functional Compensation of a Detrimental Amino Acid Substitution in the Receptor Binding Domain of the Spike Glycoprotein. J. Virol. 79: 7629-7640 [Abstract] [Full Text]  
  • Sperry, S. M., Kazi, L., Graham, R. L., Baric, R. S., Weiss, S. R., Denison, M. R. (2005). Single-Amino-Acid Substitutions in Open Reading Frame (ORF) 1b-nsp14 and ORF 2a Proteins of the Coronavirus Mouse Hepatitis Virus Are Attenuating in Mice. J. Virol. 79: 3391-3400 [Abstract] [Full Text]  
  • Coley, S. E., Lavi, E., Sawicki, S. G., Fu, L., Schelle, B., Karl, N., Siddell, S. G., Thiel, V. (2005). Recombinant Mouse Hepatitis Virus Strain A59 from Cloned, Full-Length cDNA Replicates to High Titers In Vitro and Is Fully Pathogenic In Vivo. J. Virol. 79: 3097-3106 [Abstract] [Full Text]  
  • Prentice, E., McAuliffe, J., Lu, X., Subbarao, K., Denison, M. R. (2004). Identification and Characterization of Severe Acute Respiratory Syndrome Coronavirus Replicase Proteins. J. Virol. 78: 9977-9986 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»