This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Balakireva, L. Articles by Chroboczek, J. Search for Related Content PubMed PubMed Citation Articles by Balakireva, L. Articles by Chroboczek, J.

Binding of Adenovirus Capsid to Dipalmitoyl Phosphatidylcholine Provides a Novel Pathway for Virus Entry

Larissa Balakireva,^1,* Guy Schoehn,² Eric Thouvenin,¹ and Jadwiga Chroboczek¹

Institut de Biologie Structurale Jean-Pierre Ebel, 38027 Grenoble Cedex 1,¹ EMBL Grenoble Outstation, 156X, 38042 Grenoble Cedex 9, France²

Received 28 October 2002/ Accepted 23 January 2003

Adenovirus (Ad) is an airborne, nonenveloped virus infecting respiratory epithelium. To study the mechanism of Ad entry, we used alveolar adenocarcinoma A549 cells, which have retained the ability of alveolar epithelial type II cells to synthesize the major component of pulmonary surfactant, disaturated phosphatidylcholine. Stimulation of phosphatidylcholine secretion by calcium ionophore or phorbol ester augmented the susceptibility of these cells to Ad. Both Ad infection and recombinant-Ad-mediated transfection increased in the presence of dipalmitoyl phosphatidylcholine (DPPC) liposomes in culture medium. Importantly, in the presence of DPPC liposomes, virus penetrates the cells independently of virus-specific protein receptors. DPPC vesicles bind Ad and are efficiently incorporated by A549 lung cells, serving as a virus vehicle during Ad penetration. To identify the viral protein(s) mediating Ad binding, a flotation of liposomes preincubated with structural viral proteins was employed, showing that the only Ad protein bound to DPPC vesicles was a hexon. The hexon preserved its phospholipid-binding properties upon purification, confirming its involvement in virus binding to the phospholipid. Given that disaturated phosphatidylcholine not only covers the inner surface of alveoli in the lungs but also reenters alveolar epithelium during lung surfactant turnover, Ad binding to this phospholipid may provide a pathway for virus entry into alveolar epithelium in vivo.

Permanent address: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.

This article has been cited by other articles:

• Galvan, E. M., Chen, H., Schifferli, D. M. (2007). The Psa Fimbriae of Yersinia pestis Interact with Phosphatidylcholine on Alveolar Epithelial Cells and Pulmonary Surfactant. Infect. Immun. 75: 1272-1279 [Abstract] [Full Text]  
• Johansson, C., Jonsson, M., Marttila, M., Persson, D., Fan, X.-L., Skog, J., Frangsmyr, L., Wadell, G., Arnberg, N. (2007). Adenoviruses Use Lactoferrin as a Bridge for CAR-Independent Binding to and Infection of Epithelial Cells. J. Virol. 81: 954-963 [Abstract] [Full Text]  
• Tamanini, A., Nicolis, E., Bonizzato, A., Bezzerri, V., Melotti, P., Assael, B. M., Cabrini, G. (2006). Interaction of Adenovirus Type 5 Fiber with the Coxsackievirus and Adenovirus Receptor Activates Inflammatory Response in Human Respiratory Cells. J. Virol. 80: 11241-11254 [Abstract] [Full Text]  
• Nakanishi, H., Shindou, H., Hishikawa, D., Harayama, T., Ogasawara, R., Suwabe, A., Taguchi, R., Shimizu, T. (2006). Cloning and Characterization of Mouse Lung-type Acyl-CoA:Lysophosphatidylcholine Acyltransferase 1 (LPCAT1): EXPRESSION IN ALVEOLAR TYPE II CELLS AND POSSIBLE INVOLVEMENT IN SURFACTANT PRODUCTION. J. Biol. Chem. 281: 20140-20147 [Abstract] [Full Text]  
• Zhang, Y., Bergelson, J. M. (2005). Adenovirus Receptors. J. Virol. 79: 12125-12131 [Full Text]  
• Gaden, F., Franqueville, L., Magnusson, M. K., Hong, S. S., Merten, M. D., Lindholm, L., Boulanger, P. (2004). Gene Transduction and Cell Entry Pathway of Fiber-Modified Adenovirus Type 5 Vectors Carrying Novel Endocytic Peptide Ligands Selected on Human Tracheal Glandular Cells. J. Virol. 78: 7227-7247 [Abstract] [Full Text]