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Journal of Virology, April 2003, p. 4773-4780, Vol. 77, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.8.4773-4780.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Changes in Rhinovirus Protein 2C Allow Efficient Replication in Mouse Cells

Julie R. Harris and Vincent R. Racaniello*

Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032

Received 25 October 2002/ Accepted 21 January 2003

Rhinovirus type 16 was found to replicate in mouse L cells that express the viral receptor, human intercellular adhesion molecule 1 (ICAM-1). However, infection of these cells at a low multiplicity of infection leads to no discernible cytopathic effect, and low virus titers are produced. A variant virus, 16/L, was isolated after alternate passage of rhinovirus 16 between HeLa and ICAM-1 L cells. Infection of mouse cells with 16/L leads to higher virus titers, increased production of RNA, and total cytopathic effect. Three amino acid changes were identified in the P2 region of virus 16/L, and the adaptation phenotype mapped to two changes in protein 2C. The characterization of a rhinovirus host range mutant will facilitate the investigation of cellular proteins required for efficient viral growth and the development of a murine model for rhinovirus infection.

* Corresponding author. Mailing address: Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W. 168th St., New York, NY 10032. Phone: (212) 305-5707. Fax: (212) 305-5106. E-mail: vrr1{at}columbia.edu.

Journal of Virology, April 2003, p. 4773-4780, Vol. 77, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.8.4773-4780.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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