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Journal of Virology, April 2003, p. 4463-4467, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.4463-4467.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Diverse Dengue Type 2 Virus Populations Contain Recombinant and Both Parental Viruses in a Single Mosquito Host

Scott Craig,1 Hlaing Myat Thu,1 Kym Lowry,1 Xiao-fang Wang,2 Edward C. Holmes,3 and John Aaskov1*

School of Life Sciences, Queensland University of Technology, Brisbane, Australia,1 Institute of Virology, Beijing, China,2 Department of Zoology, University of Oxford, Oxford, United Kingdom3

Received 9 September 2002/ Accepted 12 December 2002

Envelope (E) protein genes sampled from populations of dengue 2 (DEN-2) virus in individual Aedes aegypti mosquitoes and in serum from dengue patients were copied to cDNA, cloned, and sequenced. The nucleotide sequences of the E genes in more than 70% of the clones differed from the consensus sequence for the corresponding virus population at up to 11 sites, and 24 of the 94 clones contained at least one stop codon. Virus populations recovered up to 2 years apart yielded clones with similar polymorphisms in the E gene. For one mosquito, the clones obtained fell into two genotypes. One group of sequences was closely related to those of viruses recovered from dengue patients in the same locality (Yangon, Myanmar) since 1995 and were classified as Asian 1 genotype. The second group were Cosmopolitan genotype viruses which were also circulating in Yangon in 2000 and which were related to DEN-2 viruses sampled from southern China in 1999. Finally, one clone was identified as a recombinant genome composed of portions of these two "parental" genotypes. This is the first report of recombinant and parental dengue viruses in a single host.

* Corresponding author. Mailing address: School of Life Sciences, Queensland University of Technology, George St., Brisbane 4001, Australia. Phone: 61 7 3864 2144. Fax: 61 7 3864 1534. E-mail: j.aaskov{at}qut.edu.au.

Journal of Virology, April 2003, p. 4463-4467, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.4463-4467.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Aaskov, J., Buzacott, K., Field, E., Lowry, K., Berlioz-Arthaud, A., Holmes, E. C. (2007). Multiple recombinant dengue type 1 viruses in an isolate from a dengue patient. J. Gen. Virol. 88: 3334-3340 [Abstract] [Full Text]  
  • Aaskov, J., Buzacott, K., Thu, H. M., Lowry, K., Holmes, E. C. (2006). Long-Term Transmission of Defective RNA Viruses in Humans and Aedes Mosquitoes. Science 311: 236-238 [Abstract] [Full Text]  
  • Holmes, E. C. (2003). Patterns of Intra- and Interhost Nonsynonymous Variation Reveal Strong Purifying Selection in Dengue Virus. J. Virol. 77: 11296-11298 [Abstract] [Full Text]  


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