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Journal of Virology, April 2003, p. 4457-4462, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.4457-4462.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Transgenic HLA A*0201/Kb and HHD II Mice To Evaluate Frequency of Cytomegalovirus IE1-Derived Peptide Usage in Eliciting Human CD8 Cytokine Response

Department of Virology, Beckman Research Institute of the City of Hope, Duarte,¹ Department of Pediatrics, University of Southern California, Los Angeles, California²

Received 1 November 2002/ Accepted 3 January 2003

Unlike the pp65 protein of human cytomegalovirus (CMV), which has an immunodominant peptide, pp65_495-503, recognized by human CD8⁺ cells in the context of HLA A*0201, the fine peptide specificity for CMV IE1 has shown no such immunodominance. With the use of transgenic HLA A*0201/Kb and HHD II mice, a selected pool of IE1 peptides, including IE1_p256-264, IE1_p297-304, and IE1_p316-324, were shown to stimulate cytolytic T-lymphocyte lysis in the context of HLA A*0201. Based on an intracellular gamma interferon response, IE1_p297-304, a previously unrecognized CD8 epitope, triggered a prominent response to CMV IE1 in HLA A*0201 subjects.

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