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Journal of Virology, April 2003, p. 4444-4448, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.4444-4448.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Polyprotein Processing Protease of African Swine Fever Virus: Purification and Biochemical Characterization

Daniel Rubio, Alí Alejo, Irene Rodríguez, and María L. Salas*

Centro de Biología Molecular "Severo Ochoa" (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain

Received 13 September 2002/ Accepted 24 December 2002

The purified recombinant African swine fever virus polyprotein processing protease cleaves the two GG-X sites in polyprotein pp62 with the same efficiency. Cleavage at the site that is first recognized in vivo is not a requisite for cleavage at the second site, suggesting the existence of mechanisms that control the ordered processing of the polyprotein during infection.

* Corresponding author. Mailing address: Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. Phone: 34 913978478. Fax: 34 913974799. E-mail: mlsalas{at}cbm.uam.es.

Journal of Virology, April 2003, p. 4444-4448, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.4444-4448.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Eulalio, A., Nunes-Correia, I., Carvalho, A. L., Faro, C., Citovsky, V., Simoes, S., Pedroso de Lima, M. C. (2004). Two African Swine Fever Virus Proteins Derived from a Common Precursor Exhibit Different Nucleocytoplasmic Transport Activities. J. Virol. 78: 9731-9739 [Abstract] [Full Text]  
  • Ansarah-Sobrinho, C., Moss, B. (2004). Role of the I7 Protein in Proteolytic Processing of Vaccinia Virus Membrane and Core Components. J. Virol. 78: 6335-6343 [Abstract] [Full Text]  


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