Journal of Virology, April 2003, p. 3939-3949, Vol. 77, No. 7
0022-538X/03/$08.00+0 DOI: 10.1128/JVI.77.7.3939-3949.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
The Leader of Human Immunodeficiency Virus Type 1 Genomic RNA Harbors an Internal Ribosome Entry Segment That Is Active during the G2/M Phase of the Cell Cycle
Ann Brasey,1 Marcelo Lopez-Lastra,1 Theophile Ohlmann,2 Nancy Beerens,3 Ben Berkhout,3 Jean-Luc Darlix,2 and Nahum Sonenberg1*
Biochemistry Department, McGill University, H3G 1Y6 Montréal, Canada,1
LaboRetro, INSERM U 412, École Normale Supérieure de Lyon, 69364 Lyon Cedex 07, France,2
Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, 1105AZ Amsterdam, The Netherlands3
Received 10 September 2002/
Accepted 10 January 2003
The 5' leader of the human immunodeficiency virus type 1 (HIV-1) genomic RNA contains highly structured domains involved in key steps of the viral life cycle. These RNA domains inhibit cap-dependent protein synthesis. Here we report that the HIV-1 5' leader harbors an internal ribosome entry site (IRES) capable of driving protein synthesis during the G2/M cell cycle phase in which cap-dependent initiation is inhibited. The HIV-1 IRES was delineated with bicistronic mRNAs in in vitro and ex vivo assays. The HIV-1 leader IRES spans nucleotides 104 to 336 and partially overlaps the major determinants of genomic RNA packaging. These data strongly suggest that, as for HIV-1 transcription, IRES-mediated translation initiation could play an important role in virus replication during virus-induced G2/M cell cycle arrest.
* Corresponding author. Mailing address: Biochemistry Department, Room 807, McIntyre Medical Sciences Building, McGill University, 3655 Promenade Sir William Osler, H3G 1Y6 Montréal, Canada. Phone: (514) 398-7274. Fax: (514) 398-1287. E-mail: nahum.sonenberg{at}mcgill.ca.
Journal of Virology, April 2003, p. 3939-3949, Vol. 77, No. 7
0022-538X/03/$08.00+0 DOI: 10.1128/JVI.77.7.3939-3949.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Ricci, E. P., Herbreteau, C. H., Decimo, D., Schaupp, A., Datta, S. A.K., Rein, A., Darlix, J.-L., Ohlmann, T.
(2008). In vitro expression of the HIV-2 genomic RNA is controlled by three distinct internal ribosome entry segments that are regulated by the HIV protease and the Gag polyprotein. RNA
14: 1443-1455
[Abstract]
[Full Text]
-
Camerini, V., Decimo, D., Balvay, L., Pistello, M., Bendinelli, M., Darlix, J.-L., Ohlmann, T.
(2008). A Dormant Internal Ribosome Entry Site Controls Translation of Feline Immunodeficiency Virus. J. Virol.
82: 3574-3583
[Abstract]
[Full Text]
-
DeHart, J. L., Planelles, V.
(2008). Human Immunodeficiency Virus Type 1 Vpr Links Proteasomal Degradation and Checkpoint Activation. J. Virol.
82: 1066-1072
[Full Text]
-
Gendron, K., Charbonneau, J., Dulude, D., Heveker, N., Ferbeyre, G., Brakier-Gingras, L.
(2008). The presence of the TAR RNA structure alters the programmed -1 ribosomal frameshift efficiency of the human immunodeficiency virus type 1 (HIV-1) by modifying the rate of translation initiation. Nucleic Acids Res
36: 30-40
[Abstract]
[Full Text]
-
Dmitriev, S. E., Andreev, D. E., Terenin, I. M., Olovnikov, I. A., Prassolov, V. S., Merrick, W. C., Shatsky, I. N.
(2007). Efficient Translation Initiation Directed by the 900-Nucleotide-Long and GC-Rich 5' Untranslated Region of the Human Retrotransposon LINE-1 mRNA Is Strictly Cap Dependent Rather than Internal Ribosome Entry Site Mediated. Mol. Cell. Biol.
27: 4685-4697
[Abstract]
[Full Text]
-
Anderson, J. L., Johnson, A. T., Howard, J. L., Purcell, D. F. J.
(2007). Both Linear and Discontinuous Ribosome Scanning Are Used for Translation Initiation from Bicistronic Human Immunodeficiency Virus Type 1 env mRNAs. J. Virol.
81: 4664-4676
[Abstract]
[Full Text]
-
Bolinger, C., Yilmaz, A., Hartman, T. R., Kovacic, M. B., Fernandez, S., Ye, J., Forget, M., Green, P. L., Boris-Lawrie, K.
(2007). RNA helicase A interacts with divergent lymphotropic retroviruses and promotes translation of human T-cell leukemia virus type 1. Nucleic Acids Res
0: gkm124v1-14
[Abstract]
[Full Text]
-
Bjarnadottir, H., Gudmundsson, B., Gudnason, J., Jonsson, J. J.
(2006). Encapsidation Determinants Located Downstream of the Major Splice Donor in the Maedi-Visna Virus Leader Region. J. Virol.
80: 11743-11755
[Abstract]
[Full Text]
-
Cornelis, S., Tinton, S. A., Schepens, B., Bruynooghe, Y., Beyaert, R.
(2005). UNR translation can be driven by an IRES element that is negatively regulated by polypyrimidine tract binding protein. Nucleic Acids Res
33: 3095-3108
[Abstract]
[Full Text]
-
Dobson, T., Minic, A., Nielsen, K., Amiott, E., Krushel, L.
(2005). Internal initiation of translation of the TrkB mRNA is mediated by multiple regions within the 5' leader. Nucleic Acids Res
33: 2929-2941
[Abstract]
[Full Text]
-
RONFORT, C., DE BREYNE, S., SANDRIN, V., DARLIX, J.-L., OHLMANN, T.
(2004). Characterization of two distinct RNA domains that regulate translation of the Drosophila gypsy retroelement. RNA
10: 504-515
[Abstract]
[Full Text]
-
Roy, G., Miron, M., Khaleghpour, K., Lasko, P., Sonenberg, N.
(2004). The Drosophila Poly(A) Binding Protein-Interacting Protein, dPaip2, Is a Novel Effector of Cell Growth. Mol. Cell. Biol.
24: 1143-1154
[Abstract]
[Full Text]
-
Russell, R. S., Roldan, A., Detorio, M., Hu, J., Wainberg, M. A., Liang, C.
(2003). Effects of a Single Amino Acid Substitution within the p2 Region of Human Immunodeficiency Virus Type 1 on Packaging of Spliced Viral RNA. J. Virol.
77: 12986-12995
[Abstract]
[Full Text]
-
Alvarez, E., Menendez-Arias, L., Carrasco, L.
(2003). The Eukaryotic Translation Initiation Factor 4GI Is Cleaved by Different Retroviral Proteases. J. Virol.
77: 12392-12400
[Abstract]
[Full Text]
-
Meignin, C., Bailly, J.-L., Arnaud, F., Dastugue, B., Vaury, C.
(2003). The 5' Untranslated Region and Gag product of Idefix, a Long Terminal Repeat-Retrotransposon from Drosophila melanogaster, Act Together To Initiate a Switch between Translated and Untranslated States of the Genomic mRNA. Mol. Cell. Biol.
23: 8246-8254
[Abstract]
[Full Text]
Copyright © 2003 by the American Society for Microbiology. All rights reserved.