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Journal of Virology, April 2003, p. 3922-3928, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.3922-3928.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evidence that Binding of Cucumber Necrosis Virus to Vector Zoospores Involves Recognition of Oligosaccharides{dagger}

Kishore Kakani,1 Marjorie Robbins,1,{ddagger} and D'Ann Rochon2*

Faculty of Agricultural Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z4,1 Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, British Columbia V6T 1Z0, Canada2

Received 30 September 2002/ Accepted 18 December 2002

Despite the importance of vectors in natural dissemination of plant viruses, relatively little is known about the molecular features of viruses and vectors that permit their interaction in nature. Cucumber necrosis virus (CNV) is a small spherical virus whose transmission in nature is facilitated by zoospores of the fungus Olpidium bornovanus. Previous studies have shown that specific regions of the CNV capsid are involved in transmission and that transmission defects in several CNV transmission mutants are due to inefficient attachment of virions to the zoospore surface. In this study, we have undertaken to determine if zoospores contain specific receptors for CNV. We show that in vitro binding of CNV to zoospores is saturable and that vector zoospores bind CNV more efficiently than nonvector zoospores. Further studies show that treatment of zoospores with periodate and trypsin reduces CNV binding, suggesting the involvement of glycoproteins in zoospore attachment. In virus overlay assays, CNV binds to several proteins, whereas CNV transmission mutants either fail to bind or bind at significantly reduced levels. The possible involvement of specific sugars in attachment was investigated by incubating CNV with zoospores in the presence of various sugars. Two mannose derivatives (methyl {alpha}-D-mannopyranoside and D-mannosamine), as well as three mannose-containing oligosaccharides (mannotriose, {alpha}3,{alpha}6-mannopentaose, and yeast mannan) and L-(-)-fucose, all inhibited CNV binding at relatively low concentrations. Taken together, our studies suggest that binding of CNV to zoospores is mediated by specific mannose and/or fucose-containing oligosaccharides. This is the first time sugars have been implicated in transmission of a plant virus.

* Corresponding author. Mailing address: Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, British Columbia V6T 1Z0, Canada. Phone: (250) 494-6394. Fax: (250) 494-0755. E-mail: rochonda{at}agr.gc.ca.

{dagger} Agriculture and Agri-Food Canada contribution number 2172.

{ddagger} Present address: Dept. of Pathology, British Columbia Research Institute, University of British Columbia, Vancouver, BC V5Z 4H4, Canada.

Journal of Virology, April 2003, p. 3922-3928, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.3922-3928.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kakani, K., Reade, R., Katpally, U., Smith, T., Rochon, D. (2008). Induction of Particle Polymorphism by Cucumber Necrosis Virus Coat Protein Mutants In Vivo. J. Virol. 82: 1547-1557 [Abstract] [Full Text]  
  • Hui, E., Rochon, D. (2006). Evaluation of the Roles of Specific Regions of the Cucumber Necrosis Virus Coat Protein Arm in Particle Accumulation and Fungus Transmission.. J. Virol. 80: 5968-5975 [Abstract] [Full Text]  


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