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Journal of Virology, March 2003, p. 3824-3831, Vol. 77, No. 6
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.6.3824-3831.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Epstein-Barr Virus (EBV) Nuclear Antigen 1 Colocalizes with Cellular Replication Foci in the Absence of EBV Plasmids

Sayuri Ito and Kazuo Yanagi*

Herpesvirus Laboratory, Department of Virology I, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan

Received 19 September 2002/ Accepted 3 December 2002

Epstein-Barr virus (EBV) EBNA-1 is the only EBV-encoded protein that is essential for the once-per-cell-cycle replication and maintenance of EBV plasmids in latently infected cells. EBNA-1 binds to the oriP region of latent EBV plasmids and cellular metaphase chromosomes. In the absence of oriP-containing plasmids, EBNA-1 was highly colocalized with cellular DNA replication foci that were identified by immunostaining S-phase cells for proliferating cell nuclear antigen and replication protein A (RP-A) in combination with DNA short pulse-labeling. For the association of EBNA-1 with the cellular replication focus areas, the EBNA-1 regions of amino acids (aa) 8 to 94 and/or aa 315 to 410, but not the RP-A-interacting carboxy-terminal region, were necessary. These results suggest a new aspect of latent virus-cell interactions.

* Corresponding author. Mailing address: Herpesvirus Laboratory, Department of Virology I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640, Japan. Phone and fax: 81-3-5285-1180. E-mail: kyanagi{at}nih.go.jp.

Journal of Virology, March 2003, p. 3824-3831, Vol. 77, No. 6
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.6.3824-3831.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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