This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lipatov, A. S. Articles by Webster, R. G. Search for Related Content PubMed PubMed Citation Articles by Lipatov, A. S. Articles by Webster, R. G.

 Previous Article  |  Next Article 

Journal of Virology, March 2003, p. 3816-3823, Vol. 77, No. 6
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.6.3816-3823.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Neurovirulence in Mice of H5N1 Influenza Virus Genotypes Isolated from Hong Kong Poultry in 2001

Division of Virology, Department of Infectious Diseases,¹ Department of Pathology, St. Jude Children's Research Hospital,³ Department of Pathology, University of Tennessee, Memphis, Tennessee 38105,⁴ Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, People's Republic of China²

Received 30 August 2002/ Accepted 3 December 2002

We studied the pathogenicity of five different genotypes (A to E) of highly pathogenic avian H5N1 viruses, which contained HA genes similar to those of the H5N1 virus A/goose/Guangdong/1/96 and five different combinations of "internal" genes, in a mouse model. Highly pathogenic, neurotropic variants of genotypes A, C, D, and E were isolated from the brain after a single intranasal passage in mice. Genotype B virus was isolated from lungs only. The mouse brain variants had amino acid changes in all gene products except PB1, NP, and NS1 proteins but no common sets of mutations. We conclude that the original H5N1/01 isolates of genotypes A, C, D, and E were heterogeneous and that highly pathogenic neurotropic variants can be rapidly selected in mice.

This article has been cited by other articles:

• LeBouder, F., Morello, E., Rimmelzwaan, G. F., Bosse, F., Pechoux, C., Delmas, B., Riteau, B. (2008). Annexin II Incorporated into Influenza Virus Particles Supports Virus Replication by Converting Plasminogen into Plasmin. J. Virol. 82: 6820-6828 [Abstract] [Full Text]  
• Peiris, J. S. M., de Jong, M. D., Guan, Y. (2007). Avian Influenza Virus (H5N1): a Threat to Human Health. Clin. Microbiol. Rev. 20: 243-267 [Abstract] [Full Text]  
• MATEO, R. I., XIAO, S.-Y., LEI, H., DA ROSA, A. P. A. T., TESH, R. B. (2007). DHORI VIRUS (ORTHOMYXOVIRIDAE: THOGOTOVIRUS) INFECTION IN MICE: A MODEL OF THE PATHOGENESIS OF SEVERE ORTHOMYXOVIRUS INFECTION. Am J Trop Med Hyg 76: 785-790 [Abstract] [Full Text]  
• Shinya, K., Watanabe, S., Ito, T., Kasai, N., Kawaoka, Y. (2007). Adaptation of an H7N7 equine influenza A virus in mice. J. Gen. Virol. 88: 547-553 [Abstract] [Full Text]  
• Mase, M., Tanimura, N., Imada, T., Okamatsu, M., Tsukamoto, K., Yamaguchi, S. (2006). Recent H5N1 avian Influenza A virus increases rapidly in virulence to mice after a single passage in mice. J. Gen. Virol. 87: 3655-3659 [Abstract] [Full Text]  
• Tanimura, N., Tsukamoto, K., Okamatsu, M., Mase, M., Imada, T., Nakamura, K., Kubo, M., Yamaguchi, S., Irishio, W., Hayashi, M., Nakai, T., Yamauchi, A., Nishimura, M., Imai, K. (2006). Pathology of Fatal Highly Pathogenic H5N1 Avian Influenza Virus Infection in Large-billed Crows (Corvus macrorhynchos) during the 2004 Outbreak in Japan.. Vet Pathol 43: 500-509 [Abstract] [Full Text]  
• Rimmelzwaan, G. F., van Riel, D., Baars, M., Bestebroer, T. M., van Amerongen, G., Fouchier, R. A.M., Osterhaus, A. D.M.E., Kuiken, T. (2006). Influenza A Virus (H5N1) Infection in Cats Causes Systemic Disease with Potential Novel Routes of Virus Spread within and between Hosts. Am. J. Pathol. 168: 176-183 [Abstract] [Full Text]  
• Lipatov, A. S., Andreansky, S., Webby, R. J., Hulse, D. J., Rehg, J. E., Krauss, S., Perez, D. R., Doherty, P. C., Webster, R. G., Sangster, M. Y. (2005). Pathogenesis of Hong Kong H5N1 influenza virus NS gene reassortants in mice: the role of cytokines and B- and T-cell responses. J. Gen. Virol. 86: 1121-1130 [Abstract] [Full Text]  
• Govorkova, E. A., Rehg, J. E., Krauss, S., Yen, H.-L., Guan, Y., Peiris, M., Nguyen, T. D., Hanh, T. H., Puthavathana, P., Long, H. T., Buranathai, C., Lim, W., Webster, R. G., Hoffmann, E. (2005). Lethality to Ferrets of H5N1 Influenza Viruses Isolated from Humans and Poultry in 2004. J. Virol. 79: 2191-2198 [Abstract] [Full Text]  
• Lipatov, A. S., Govorkova, E. A., Webby, R. J., Ozaki, H., Peiris, M., Guan, Y., Poon, L., Webster, R. G. (2004). Influenza: Emergence and Control. J. Virol. 78: 8951-8959 [Full Text]  
• Guan, Y., Poon, L. L. M., Cheung, C. Y., Ellis, T. M., Lim, W., Lipatov, A. S., Chan, K. H., Sturm-Ramirez, K. M., Cheung, C. L., Leung, Y. H. C., Yuen, K. Y., Webster, R. G., Peiris, J. S. M. (2004). H5N1 influenza: A protean pandemic threat. Proc. Natl. Acad. Sci. USA 101: 8156-8161 [Abstract] [Full Text]  
• Sturm-Ramirez, K. M., Ellis, T., Bousfield, B., Bissett, L., Dyrting, K., Rehg, J. E., Poon, L., Guan, Y., Peiris, M., Webster, R. G. (2004). Reemerging H5N1 Influenza Viruses in Hong Kong in 2002 Are Highly Pathogenic to Ducks. J. Virol. 78: 4892-4901 [Abstract] [Full Text]